Associated products


Our Abpromise guarantee covers the use of ab5199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 160, 90 kDa (predicted molecular weight: 153 kDa).Can be blocked with Human KDM2B peptide (ab23079).


  • FunctionHistone demethylase that demethylates 'Lys-4' and 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' and dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. Preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which inhibits cell growth and proliferation. May also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
  • Sequence similaritiesBelongs to the JHDM1 histone demethylase family.
    Contains 1 CXXC-type zinc finger.
    Contains 1 F-box domain.
    Contains 1 JmjC domain.
    Contains 7 LRR (leucine-rich) repeats.
    Contains 1 PHD-type zinc finger.
  • DomainThe JmjC domain mediates demethylation activity (By similarity). It is also required for repression of ribosomal RNA genes.
  • Cellular localizationNucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • [Histone-H3]-lysine-36 demethylase 1B antibody
    • CXXC-type zinc finger protein 2 antibody
    • CXXC2 antibody
    • F box and leucine rich repeat protein 10 antibody
    • F box protein FBL10 antibody
    • F-box and leucine-rich repeat protein 10 antibody
    • F-box protein FBL10 antibody
    • F-box/LRR-repeat protein 10 antibody
    • Fbl10 antibody
    • FBXL10 antibody
    • JEMMA (Jumonji domain EMSY interactor methyltransferase motif) protein antibody
    • JHDM1B antibody
    • JmjC domain-containing histone demethylation protein 1B antibody
    • Jumonji C domain containing histone demethylase 1B antibody
    • Jumonji domain-containing EMSY-interactor methyltransferase motif protein antibody
    • kdm2b antibody
    • KDM2B_HUMAN antibody
    • Lysine (K) specific demethylase 2B antibody
    • Lysine-specific demethylase 2B antibody
    • PCCX2 antibody
    • Protein containing CXXC domain 2 antibody
    • Protein JEMMA antibody
    • Protein-containing CXXC domain 2 antibody
    see all

Anti-KDM2B antibody images

  • Anti-KDM2B antibody (ab5199) + HeLa whole cell lysates

    HRP-conjugated donkey anti-goat secondary antibody (chemicon international)

    Predicted band size : 153 kDa
    Observed band size : ~ 160,~ 90 kDa (why is the actual band size different from the predicted?)

    Exposure time : 10 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-KDM2B antibody (ab5199)

This product has been referenced in:
  • Tzatsos A  et al. Lysine-specific Demethylase 2B (KDM2B)-let-7-Enhancer of Zester Homolog 2 (EZH2) Pathway Regulates Cell Cycle Progression and Senescence in Primary Cells. J Biol Chem 286:33061-9 (2011). WB ; Mouse . Read more (PubMed: 21757686) »
  • Ge R  et al. F-box protein 10, an NF-?B-dependent anti-apoptotic protein, regulates TRAIL-induced apoptosis through modulating c-Fos/c-FLIP pathway. Cell Death Differ 18:1184-95 (2011). Read more (PubMed: 21252908) »

See all 2 Publications for this product

Product Wall

Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this product. As our Abpromise indicates, in the event that a pro...

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Application Western blot
Sample Mouse Cell lysate - other (NIH3T3, Neuro2A and ES)
Loading amount 20 µg
Specification NIH3T3, Neuro2A and ES
Gel Running Conditions Reduced Denaturing (8)
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Jun 28 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 100 µg
Specification HeLa cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%

Abcam user community

Verified customer

Submitted Jul 26 2006