Recombinant fragment (Human)
Our Abpromise guarantee covers the use of ab91252 in the following tested applications.
|ChIP||Use at an assay dependent concentration. PubMed: 23386205|
|Flow Cyt||Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000. Predicted molecular weight: 147 kDa.|
|ELISA||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
IHC image of KDM3A / JHDM2A antibody staining in Human normal spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91252, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.