Overview

  • Product nameAnti-KDM3A / JHDM2A antibody
    See all KDM3A / JHDM2A primary antibodies
  • Description
    Rabbit polyclonal to KDM3A / JHDM2A
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1271-1321 of human KDM3A/ JHDM2A

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab80598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 2-5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 147 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionHistone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
    • Sequence similaritiesBelongs to the JHDM2 histone demethylase family.
      Contains 1 JmjC domain.
    • DomainThe JmjC domain and the C6-type zinc-finger are required for the demethylation activity.
      Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors.
    • Cellular localizationCytoplasm. Nucleus. Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp686A24246 antibody
      • DKFZp686P07111 antibody
      • JHDM2A antibody
      • JHMD2A antibody
      • JmjC domain-containing histone demethylation protein 2A antibody
      • JMJD1 antibody
      • JMJD1A antibody
      • Jumonji C domain containing histone demethylase 2A antibody
      • Jumonji domain containing 1 antibody
      • Jumonji domain containing 1A antibody
      • Jumonji domain containing protein 1A antibody
      • Jumonji domain-containing protein 1A antibody
      • Kdm3a antibody
      • KDM3A_HUMAN antibody
      • KIAA0742 antibody
      • Lysine (K) specific demethylase 3A antibody
      • Lysine-specific demethylase 3A antibody
      • Testis specific protein A antibody
      • TSGA antibody
      see all

    Anti-KDM3A / JHDM2A antibody images

    • All lanes : Anti-KDM3A / JHDM2A antibody (ab80598) at 0.1 µg/ml

      Lane 1 : whole HeLa cell lysate at 50 µg
      Lane 2 : whole HeLa cell lysate at 15 µg
      Lane 3 : whole HeLa cell lysate at 5 µg


      Predicted band size : 147 kDa
      Observed band size : 171 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 300 kDa,85 kDa. We are unsure as to the identity of these extra bands.
    • Detection of Human KDM3A / JHDM2A by Immunoprecipitation. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Lane 1: ab80598 used for IP at 3 µg/mg lysate; Lane 2: a control IgG was used for IP. For subsequent Western blot detection, ab80598 was used at 1 µg/ml.

    References for Anti-KDM3A / JHDM2A antibody (ab80598)

    This product has been referenced in:
    • Hickok JR  et al. Nitric oxide modifies global histone methylation by inhibiting Jumonji C domain-containing demethylases. J Biol Chem 288:16004-15 (2013). WB ; Human . Read more (PubMed: 23546878) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Rat Cell lysate - whole cell (Rat Trophoblast stem cell and human trophoblast ce)
    Loading amount 100 µg
    Specification Rat Trophoblast stem cell and human trophoblast ce
    Treatment 0.5% oxygen for 24 hours
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Verified customer

    Submitted Jan 27 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"