Anti-KDM3A / JHDM2A antibody - Aminoterminal end (ab75620)


  • Product nameAnti-KDM3A / JHDM2A antibody - Aminoterminal end
    See all KDM3A / JHDM2A primary antibodies
  • Description
    Rabbit polyclonal to KDM3A / JHDM2A - Aminoterminal end
  • Tested applicationsSuitable for: ICC/IF, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH conjugated synthetic peptide selected from the N terminal region of human KDM3A/ JHDM2A.

  • Positive control
    • Human hepatocarcinoma tissue



Our Abpromise guarantee covers the use of ab75620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
ELISA 1/1000.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.


  • FunctionHistone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
  • Sequence similaritiesBelongs to the JHDM2 histone demethylase family.
    Contains 1 JmjC domain.
  • DomainThe JmjC domain and the C6-type zinc-finger are required for the demethylation activity.
    Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors.
  • Cellular localizationCytoplasm. Nucleus. Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686A24246 antibody
    • DKFZp686P07111 antibody
    • JHDM2A antibody
    • JHMD2A antibody
    • JmjC domain-containing histone demethylation protein 2A antibody
    • JMJD1 antibody
    • JMJD1A antibody
    • Jumonji C domain containing histone demethylase 2A antibody
    • Jumonji domain containing 1 antibody
    • Jumonji domain containing 1A antibody
    • Jumonji domain containing protein 1A antibody
    • Jumonji domain-containing protein 1A antibody
    • Kdm3a antibody
    • KDM3A_HUMAN antibody
    • KIAA0742 antibody
    • Lysine (K) specific demethylase 3A antibody
    • Lysine-specific demethylase 3A antibody
    • Testis specific protein A antibody
    • TSGA antibody
    see all

Anti-KDM3A / JHDM2A antibody - Aminoterminal end images

  • ab75620, at a dilution of 1/50, staining KDM3A / JHDM2A in formalin fixed and paraffin embedded human hepatocarcinoma tissue by Immunohistochemistry. A peroxidase conjugated secondary antibody was then used, followed by DAB staining.
  • ICC/IF image of ab75620 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75620, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-KDM3A / JHDM2A antibody - Aminoterminal end (ab75620)

This product has been referenced in:
  • Du ZM  et al. Upregulation of MiR-155 in Nasopharyngeal Carcinoma is Partly Driven by LMP1 and LMP2A and Downregulates a Negative Prognostic Marker JMJD1A. PLoS One 6:e19137 (2011). IHC-P ; Human . Read more (PubMed: 21541331) »

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