• Product name
    Anti-KDM3B / JMJD1B antibody
    See all KDM3B / JMJD1B primary antibodies
  • Description
    Rabbit polyclonal to KDM3B / JMJD1B
  • Host species
  • Tested applications
    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
  • Immunogen

    Synthetic peptide corresponding to Human KDM3B/ JMJD1B (C terminal).
    Database link: Q7LBC6

  • Positive control
    • HeLa and 293T whole cell lysates. IHC Controls: Laryngeal squamous cell carcinoma, Ovarian carcinoma, Prostate carcinoma



Our Abpromise guarantee covers the use of ab70797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IF 1/50 - 1/250.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 240 kDa (predicted molecular weight: 191 kDa).
IP Use at 2-5 µg/mg of lysate.


  • Function
    Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity.
  • Tissue specificity
    Ubiquitous. Highly expressed in placenta, skeletal muscle, kidney, heart and liver.
  • Sequence similarities
    Belongs to the JHDM2 histone demethylase family.
    Contains 1 JmjC domain.
  • Domain
    Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • 5qNCA antibody
    • C5orf7 antibody
    • JHDM2B antibody
    • JmjC domain containing histone demethylation protein 2B antibody
    • JmjC domain-containing histone demethylation protein 2B antibody
    • jmjd1b antibody
    • Jumonji domain containing 1B antibody
    • Jumonji domain containing protein 1B antibody
    • Jumonji domain-containing protein 1B antibody
    • KDM3B antibody
    • KDM3B_HUMAN antibody
    • KIAA1082 antibody
    • Lysine (K) specific demethylase 3B antibody
    • Lysine-specific demethylase 3B antibody
    • NET22 antibody
    • Nuclear protein 5qNCA antibody
    see all


  • All lanes : Anti-KDM3B / JMJD1B antibody (ab70797) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 191 kDa
    Observed band size: 240 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 110 kDa, 30 kDa, 60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 3 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling KDM3B / JMJD1B with ab70797 at 1/200 (1µg/ml). Detection: DAB.
  • Detection of KDM3B / JMJD1B by Western Blot of Immunoprecipitate.
    ab70797 at 1µg/ml staining KDM3B / JMJD1B in HeLa whole cell lysate immunoprecipitated using ab70797 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.


This product has been referenced in:
  • Zepp M  et al. IDK1 is a rat monoclonal antibody against hypoglycosylated bone sialoprotein with application as biomarker and therapeutic agent in breast cancer skeletal metastasis. J Pathol Clin Res 4:55-68 (2018). Read more (PubMed: 29416877) »
  • Liu Y  et al. An epigenetic role for PRL-3 as a regulator of H3K9 methylation in colorectal cancer. Gut : (2012). Human . Read more (PubMed: 22345654) »

See all 3 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Mouse Cell lysate - whole cell (mouse embryonic stem cells)
Gel Running Conditions
Reduced Denaturing (6% Gel)
Loading amount
500000 cells
mouse embryonic stem cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

Submitted Nov 01 2016


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