Anti-KDM4A / JHDM3A / JMJD2A antibody (ab106472)

Overview

  • Product nameAnti-KDM4A / JHDM3A / JMJD2A antibody
    See all KDM4A / JHDM3A / JMJD2A primary antibodies
  • Description
    Rabbit polyclonal to KDM4A / JHDM3A / JMJD2A
  • Specificityab106472 is specific for JMJD2A and will not recognize the other JMJD proteins.
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 14 amino acid synthetic peptide from near the center of Human KDM4A/ JHDM3A/ JMJD2A (CAH71021).

  • Positive control
    • Rat liver tissue lysate; Rat liver tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab106472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 121 kDa.

Target

  • FunctionHistone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.
    Isoform 2: Crucial for muscle differentiation, promotes transcriptional activation of the Myog gene by directing the removal of repressive chromatin marks at its promoter. Lacks the N-terminal demethylase domain.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the JHDM3 histone demethylase family.
    Contains 1 C2HC pre-PHD-type zinc finger.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
    Contains 2 PHD-type zinc fingers.
    Contains 2 Tudor domains.
  • DomainThe 2 Tudor domains recognize and bind methylated histone H3 'Lys-4' residue (H3K4me). Double Tudor domain has an interdigitated structure and the unusual fold is required for its ability to bind methylated histone tails. Trimethylated H3 'Lys-4' (H3K4me3) is bound in a cage of 3 aromatic residues, 2 of which are from the Tudor domain 2, while the binding specificity is determined by side-chain interactions involving residues from the Tudor domain 1. The Tudor domains are also able to bind trimethylated histone H3 'Lys-9' (H3K9me3), di- and trimethylated H4 'Lys-20' (H4K20me2 and H4K20me3). Has high affinity for H4K20me2, blocking recruitment of proteins such as TP53BP1.
  • Post-translational
    modifications
    Ubiquitinated by RNF8 and RNF168 following DNA damage, leading to its degradation. Degradation promotes accessibility of H4K20me2 mark for DNA repair protein TP53BP1, which is then recruited.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • JHDM3A antibody
    • JmjC domain containing histone demethylation protein 3A antibody
    • JmjC domain-containing histone demethylation protein 3A antibody
    • JMJD2 antibody
    • JMJD2A antibody
    • jumonji C domain containing histone demethylase 3A antibody
    • Jumonji domain containing 2 antibody
    • Jumonji domain containing 2A antibody
    • Jumonji domain containing protein 2A antibody
    • Jumonji domain-containing protein 2A antibody
    • KDM4A antibody
    • KDM4A_HUMAN antibody
    • KIAA0677 antibody
    • Lysine (K) specific demethylase 4A antibody
    • Lysine-specific demethylase 4A antibody
    • TDRD14A antibody
    • Tudor domain containing 14A antibody
    see all

Anti-KDM4A / JHDM3A / JMJD2A antibody images

  • Lane 1 : Anti-KDM4A / JHDM3A / JMJD2A antibody (ab106472) at 1 µg/ml
    Lane 2 : Anti-KDM4A / JHDM3A / JMJD2A antibody (ab106472) at 2 µg/ml

    Lane 1 : Rat liver tissue lysate
    Lane 2 : Rat liver tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 121 kDa
  • ab106472, at 5 µg/ml, staining KDM4A / JHDM3A / JMJD2A in rat liver tissue by Immunohistochemistry.

References for Anti-KDM4A / JHDM3A / JMJD2A antibody (ab106472)

ab106472 has not yet been referenced specifically in any publications.

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