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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab27531? Please let us know so that we can cite the reference in this datasheet
ab27531 has been referenced in 2 publications.
- Liu Y et al. An epigenetic role for PRL-3 as a regulator of H3K9 methylation in colorectal cancer. Gut : (2012). WB, ICC/IF; Human. PubMed: 22345654
- Beyer S et al. The histone demethylases JMJD1A and JMJD2B are transcriptional targets of hypoxia-inducible factor HIF. J Biol Chem 283:36542-52 (2008). WB; Human. PubMed: 18984585
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - KDM4B / JMJD2B antibody (ab27531)
All lanes : Anti-KDM4B / JMJD2B antibody (ab27531) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 :
Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Nuclear Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 121 kDa
Observed band size : 150 kDa (why is the actual band size different from the predicted?)
Additional bands at : 105 kDa,55 kDa,80 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
The 150 kDa band observed is comparable to molecular weights seen with other commercially available antibodies to KDM4B/JMJD2B.
Immunocytochemistry/ Immunofluorescence - Anti-KDM4B / JMJD2B antibody (ab27531)
ICC/IF image of ab27531 stained T47D cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab27531 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
JMJD2B antibody for WB in Mouse (27531)
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