Overview

  • Product nameAnti-KDM4B / JMJD2B antibodySee all KDM4B / JMJD2B primary antibodies ...
  • Description
    Rabbit polyclonal to KDM4B / JMJD2B
  • Tested applicationsWB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human JMJD2B.

    (Peptide available as ab30598.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: Jurkat Whole Cell; Jurkat Nuclear; K562 Whole Cell and K562 Nuclear. This antibody gave a positive signal in IF/ICC in the following methanol fixed cell line: T47D.

Properties

Applications

Our Abpromise guarantee covers the use of ab27531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 121 kDa).Can be blocked with Human KDM4B / JMJD2B peptide (ab30598).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • RelevanceKDM4B / JMJD2B is a jumonji domain containing histone demethylase that specifically demethylates histone H3 at K9 and K36.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • JHDM3B antibody
    • JmjC domain-containing histone demethylation protein 3B antibody
    • JMJD2B antibody
    • Jumonji domain containing protein 2B antibody
    • Jumonji domain-containing protein 2B antibody
    • KIAA0876 antibody
    • Lysine-specific demethylase 4B antibody
    see all

Anti-KDM4B / JMJD2B antibody images

  • All lanes : Anti-KDM4B / JMJD2B antibody (ab27531) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)
    Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Nuclear Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 121 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 105 kDa,55 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • ICC/IF image of ab27531 stained T47D cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab27531 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-KDM4B / JMJD2B antibody (ab27531)

This product has been referenced in:
  • Liu Y  et al. An epigenetic role for PRL-3 as a regulator of H3K9 methylation in colorectal cancer. Gut : (2012). WB, ICC/IF ; Human . Read more (PubMed: 22345654) »
  • Beyer S  et al. The histone demethylases JMJD1A and JMJD2B are transcriptional targets of hypoxia-inducible factor HIF. J Biol Chem 283:36542-52 (2008). WB ; Human . Read more (PubMed: 18984585) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10% tris glycine gel)
Sample Human Cell lysate - whole cell (rhabdomyosarcoma cell lines)
Specification rhabdomyosarcoma cell lines
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jul 31 2013

Thank you for contacting us.

In regards to the homology observed between the sequence provided and the immunogen used to raise the antibodies mentioned, the highest percentage corresponds to ab91549, which shares 75% of the sequence.
Read More

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblast)
Loading amount 50 µg
Specification Mouse embryonic fibroblast
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 05 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"