Overview

  • Product name
    Anti-KDM4B / JMJD2B antibody
    See all KDM4B / JMJD2B primary antibodies
  • Description
    Rabbit polyclonal to KDM4B / JMJD2B
  • Tested applications
    Suitable for: Flow Cyt, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human KDM4B/ JMJD2B aa 4-31 (N terminal) conjugated to keyhole limpet haemocyanin.
    Database link: O94953

  • Positive control
    • K562 cell lysate, Human Testis tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab103129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.
WB 1/100 - 1/500. Predicted molecular weight: 122 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate.
  • Sequence similarities
    Belongs to the JHDM3 histone demethylase family.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
    Contains 2 PHD-type zinc fingers.
    Contains 2 Tudor domains.
  • Domain
    The 2 Tudor domains recognize and bind methylated histones. Double Tudor domain has an interdigitated structure and the unusual fold is required for its ability to bind methylated histone tails.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • JHDM3B antibody
    • JmjC domain-containing histone demethylation protein 3B antibody
    • JMJD2B antibody
    • Jumonji domain containing 2B antibody
    • Jumonji domain containing protein 2B antibody
    • Jumonji domain-containing protein 2B antibody
    • Kdm4b antibody
    • KDM4B_HUMAN antibody
    • KIAA0876 antibody
    • Lysine (K) specific demethylase 4B antibody
    • Lysine demethylase 4B antibody
    • Lysine-specific demethylase 4B antibody
    • TDRD14B antibody
    • Tudor domain containing 14B antibody
    see all

Images

  • Anti-KDM4B / JMJD2B antibody (ab103129) at 1/100 dilution + K562 cell lysate at 35 µg

    Predicted band size : 122 kDa
  • Formalin-fixed and paraffin-embedded Human Testis tissue, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining, using ab103129 at a dilution of 1/50.
  • Flow cytometry analysis of K562 cellls (right histogram) compared to a negative control cell (left histogram) using ab103129.FITC-conjugated goat anti-rabbit secondary antibodies were used for the analysis.

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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