Anti-KDM4B / JMJD2B antibody (ab91549)

Overview

  • Product nameAnti-KDM4B / JMJD2B antibody
    See all KDM4B / JMJD2B primary antibodies
  • Description
    Rabbit polyclonal to KDM4B / JMJD2B
  • Specificityab91549 is specific for JMJD2B and will not recognize the other JMJD proteins.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human KDM4B/ JMJD2B aa 360-410 (internal sequence).
    Database link: O94953

  • Positive control
    • A20 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab91549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 122 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionHistone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate.
  • Sequence similaritiesBelongs to the JHDM3 histone demethylase family.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
    Contains 2 PHD-type zinc fingers.
    Contains 2 Tudor domains.
  • DomainThe 2 Tudor domains recognize and bind methylated histones. Double Tudor domain has an interdigitated structure and the unusual fold is required for its ability to bind methylated histone tails.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • JHDM3B antibody
    • JmjC domain-containing histone demethylation protein 3B antibody
    • JMJD2B antibody
    • Jumonji domain containing protein 2B antibody
    • Jumonji domain-containing protein 2B antibody
    • Kdm4b antibody
    • KDM4B_HUMAN antibody
    • KIAA0876 antibody
    • Lysine-specific demethylase 4B antibody
    see all

Anti-KDM4B / JMJD2B antibody images

  • Lane 1 : Anti-KDM4B / JMJD2B antibody (ab91549) at 1 µg/ml
    Lane 2 : Anti-KDM4B / JMJD2B antibody (ab91549) at 2 µg/ml

    Lane 1 : A20 cell lysate
    Lane 2 : A20 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 122 kDa

References for Anti-KDM4B / JMJD2B antibody (ab91549)

ab91549 has not yet been referenced specifically in any publications.

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Unfortunately for this antibody we have data for WB and ELISA only and I am sure these applications does not involve fixing. The no data however does not mean that antibody is not suitable, it might be suitable how...

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In regards to the homology observed between the sequence provided and the immunogen used to raise the antibodies mentioned, the highest percentage corresponds to ab91549, which shares 75% of the sequence.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"