Anti-KDM4C / GASC1 / JMJD2C antibody (ab85454)

Overview

  • Product nameAnti-KDM4C / GASC1 / JMJD2C antibody
    See all KDM4C / GASC1 / JMJD2C primary antibodies
  • Description
    Rabbit polyclonal to KDM4C / GASC1 / JMJD2C
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence amino acids 755-804 (CLCNLRGGAL KQTKNNKWAH VMCAVAVPEV RFTNVPERTQ IDVGRIPLQR) of Human KDM4C/ GASC1/ JMJD2C, NP_055876.

  • Positive control
    • HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab85454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 120 kDa).Can be blocked with Human KDM4C / GASC1 / JMJD2C peptide (ab111662) or KDM4C / GASC1 / JMJD2C peptide (ab155886). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionHistone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate.
  • Tissue specificityOverexpressed in several esophageal squamous cell carcinomas (ESCs).
  • Sequence similaritiesBelongs to the JHDM3 histone demethylase family.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
    Contains 2 PHD-type zinc fingers.
    Contains 2 Tudor domains.
  • DomainThe 2 Tudor domains recognize and bind methylated histones. Double Tudor domain has an interdigitated structure and the unusual fold is required for its ability to bind methylated histone tails.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA146B14.1 antibody
    • GASC 1 protein antibody
    • GASC-1 protein antibody
    • GASC1 antibody
    • Gene amplified in squamous cell carcinoma 1 protein antibody
    • JHDM3C antibody
    • JmjC domain containing histone demethylation protein 3C antibody
    • JmjC domain-containing histone demethylation protein 3C antibody
    • JMJD2C antibody
    • Jumonji domain containing 2C antibody
    • Jumonji domain containing protein 2C antibody
    • Jumonji domain-containing protein 2C antibody
    • Kdm4c antibody
    • KDM4C_HUMAN antibody
    • Lysine (K) specific demethylase 4C antibody
    • Lysine-specific demethylase 4C antibody
    • TDRD14C antibody
    • Tudor domain containing 14C antibody
    see all

Anti-KDM4C / GASC1 / JMJD2C antibody images

  • Anti-KDM4C / GASC1 / JMJD2C antibody (ab85454) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 120 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)

References for Anti-KDM4C / GASC1 / JMJD2C antibody (ab85454)

This product has been referenced in:
  • Burton A  et al. Inositol pyrophosphates regulate JMJD2C-dependent histone demethylation. Proc Natl Acad Sci U S A 110:18970-5 (2013). Read more (PubMed: 24191012) »
  • Lu C  et al. IDH mutation impairs histone demethylation and results in a block to cell differentiation. Nature 483:474-8 (2012). Read more (PubMed: 22343901) »

See all 2 Publications for this product

Product Wall

I have a follow up question based on multiple bands disappearing when the blocking peptide is added. What are the molecular weights of the other bands in your blot? If more than one band disappears in Western blot by peptide/antigen competition, those ...

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Thank you for your reply.

I have sent you the blocking peptide (http://www.abcam.com/kdm4c--gasc1--jmjd2c-peptide-ab155886.html) free of charge. Your order number is 1236828, and is expected to be delivered Friday, 1/18/2013.

Below is...

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Knockdown at the mRNA level does not necessarily equate to a visible difference at the protein level in the WB band intensity. Since 100kDa is the expected band size for KDM4C, it is very likely that this band is not non-specific but rather a refl...

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Vielen Dank für Ihre Antwort.

Die Referenznummer Ihrer Gutschrift ist 123456

Bitte setzen Sie sich mit der Apotheke/Reagenzienzentrale in Kontakt, um zu verhindern, dass diese Ihre Gutschrift/die Erstattung ohne Ihr Einverst&a...

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Leider hat sich mein Verdacht bestätigt: der Antikörper wurde noch nicht in der Maus getestet. ich habe unser Datenblatt dementsprechend korrigiert.

Es ist übrigens sehr interessant, dass der Antikörper nicht kreuzreagiert, da die beiden Pro...

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I am very pleased to hear you would like to accept our offer and test these antibodies noted above in IP. This code will give you: 1 free PRIMARY ANTIBODY OR COST OF PRODUCT OFF OF ORDER before the expiration date. To redeem this offer, please sub...

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Thank you for your message and for getting back to me with further details. It is much appreciated.

I am sorry to hear you are disappointed with our products. I appreciate the time you have spent on these experiments in the laboratory and it ...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will provide us with vital informa...

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I have checked our system which stores all the incoming and outgoing e-mails and I can see that in the initial correspondence, 3 products (ab47535, ab71732 and ab85454) were reported to be faulty.

Could ...

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Thank you for your response.

My colleague is out of office and she has asked me to look after her customers. I could arrange a credit note for you which you can use in the future.

Please do let me know how you wish to proceed with y...

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