Our Abpromise guarantee covers the use of ab85454 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 120 kDa).Can be blocked with Human KDM4C / GASC1 / JMJD2C peptide (ab111662) or KDM4C / GASC1 / JMJD2C peptide (ab155886). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|