Overview

  • Product nameAnti-KDM4D / JMJD2D antibody
    See all KDM4D / JMJD2D primary antibodies
  • Description
    Rabbit polyclonal to KDM4D / JMJD2D
  • Tested applicationsSuitable for: WB, IP, Flow Cyt, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-354 of Human KDM4D/ JMJD2D (43kDa).

  • Positive control
    • DLD1 (Human colorectal adenocarcinoma) cells; DLD1 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab93694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200. Predicted molecular weight: 58 kDa.
IP Use at 20 µg/mg of lysate.
Flow Cyt Use a concentration of 5 µg/ml.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionHistone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Demethylates both di- and trimethylated H3 'Lys-9' residue, while it has no activity on monomethylated residues. Demethylation of Lys residue generates formaldehyde and succinate.
  • Sequence similaritiesBelongs to the JHDM3 histone demethylase family.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • JHD3D antibody
    • JHDM3D antibody
    • JmjC domain-containing histone demethylation protein 3D antibody
    • Jumonji domain-containing protein 2D antibody
    • Kdm4d antibody
    • KDM4D_HUMAN antibody
    • Lysine-specific demethylase 4D antibody
    see all

Anti-KDM4D / JMJD2D antibody images

  • ab93694, at 10µg/ml, staining KDM4D / JMJD2D in DLD1 (Human Colorectal Adenocarcinoma) cells by Immunofluorescence. Cells were fixed with 1% formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with normal goat serum. Secondary antibody: Goat anti Rabbit Cy3 at 1/250 dilution.
  • All lanes : Anti-KDM4D / JMJD2D antibody (ab93694) at 1/200 dilution

    Lane 1 : DLD1 cell lysate at 10 µg
    Lane 2 : DLD1 cell lysate at 5 µg


    Predicted band size : 58 kDa
  • IHC image of ab93694 staining in Human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93694, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-KDM4D / JMJD2D antibody (ab93694)

This product has been referenced in:
  • Badal S  et al. Nonantibiotic Effects of Fluoroquinolones in Mammalian Cells. J Biol Chem 290:22287-97 (2015). WB ; Human . Read more (PubMed: 26205818) »
  • Khoury-Haddad H  et al. PARP1-dependent recruitment of KDM4D histone demethylase to DNA damage sites promotes double-strand break repair. Proc Natl Acad Sci U S A 111:E728-37 (2014). WB, ICC/IF ; Human . Read more (PubMed: 24550317) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"