KDM4/JMJD2 (Lysine-specific demethylase 4) is a family of histone demethyltransferases involved in H3(meK9) specific demethylation. The KDM4/JMJD2 family are JmjC proteins that specifically removes tri-methylation from H3(meK9) and H3(meK36) but has no activity on mono- or di-methylated residues.
Abcam's KDM4/JMJD2 Activity Quantification Assay Kit (Fluorometric) (ab113462) is designed for the easy and fast fluorometric measurement of KDM4/JMJD2 activity or inhibition. The antibody-based, immunospecific method directly detects KDM4/JMJD2-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.
|Components||48 tests||96 tests|
|10X Wash Buffer||1 x 14ml||1 x 28ml|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|Adhesive Covering Film||1 unit||1 unit|
|Capture Antibody, 1000 µg/mL||1 x 5µl||1 x 10µl|
|Co-factor 1||1 x 30µl||1 x 60µl|
|Co-factor 2||1 x 30µl||1 x 60µl|
|Co-factor 3||1 x 30µl||1 x 60µl|
|Detection Antibody, 400 µg/mL||1 x 6µl||1 x 12µl|
|Fluoro Developer||1 x 10µl||1 x 20µl|
|Fluoro Dilution||1 x 4ml||1 x 8ml|
|Fluoro Enhancer||1 x 10µl||1 x 20µl|
|JMJD2 Assay Buffer||1 x 4ml||1 x 8ml|
|JMJD2 Assay Standard, 50 µg/mL||1 x 10µl||1 x 20µl|
|JMJD2 Substrate, 50 µg/mL||1 x 60µl||1 x 120µl|
Our Abpromise guarantee covers the use of ab113462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|