Anti-KDM5A / Jarid1A / RBBP2 antibody (ab92533)
Key features and details
- Rabbit polyclonal to KDM5A / Jarid1A / RBBP2
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KDM5A / Jarid1A / RBBP2 antibody
See all KDM5A / Jarid1A / RBBP2 primary antibodies -
Description
Rabbit polyclonal to KDM5A / Jarid1A / RBBP2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Dog, Gorilla, Chinese hamster, Orangutan -
Immunogen
Synthetic peptide corresponding to Human KDM5A/ Jarid1A/ RBBP2 aa 1640-1690.
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Positive control
- Human prostate carcinoma.
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General notes
Concentration is optimized for IHC and not determined
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500.
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Notes |
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IHC-P
1/100 - 1/500. |
Target
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Function
Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. May stimulate transcription mediated by nuclear receptors. May be involved in transcriptional regulation of Hox proteins during cell differentiation. May participate in transcriptional repression of cytokines such as CXCL12. -
Sequence similarities
Belongs to the JARID1 histone demethylase family.
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 3 PHD-type zinc fingers. -
Domain
The GSGFP motif is required for the interaction with SUZ12. -
Cellular localization
Nucleus > nucleolus. Occupies promoters of genes involved in RNA metabolism and mitochondrial function. - Information by UniProt
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Database links
- Entrez Gene: 5927 Human
- Entrez Gene: 312678 Rat
- Omim: 180202 Human
- SwissProt: P29375 Human
- Unigene: 76272 Human
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Alternative names
- Histone demethylase JARID1A antibody
- JARID1A antibody
- Jumonji/ARID domain containing protein 1A antibody
see all
Images
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ab92533 at 1/250 dilution staining KDM5A / Jarid1A / RBBP2 in human prostate carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
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Immunohistochemical analysis of parafin embedded human breast carcinoma tissue labelling KDM5A/Jarid1A/RBBP2 with ab92533 at 1 in 250 dilution.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab92533 has been referenced in 1 publication.
- Du C et al. Activation of the KDM5A/miRNA-495/YTHDF2/m6A-MOB3B axis facilitates prostate cancer progression. J Exp Clin Cancer Res 39:223 (2020). PubMed: 33087165