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Anti-KDM5B / PLU1 / Jarid1B antibody - ChIP Grade (ab45301)

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Overview

Product name

Anti-KDM5B / PLU1 / Jarid1B antibody - ChIP Grade
See all KDM5B / PLU1 / Jarid1B products (6) ...

Description

Rabbit polyclonal to KDM5B / PLU1 / Jarid1B - ChIP Grade

Specificity

This antibody detects Retinoblastoma binding protein 2 homolog 1 only.

Tested applications

IHC-P, IP, ChIPmore details

Cross reactivity

Reacts with

Human

Immunogen

Protein fragment of KDM5B.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Rb family
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
Epigenetics and Nuclear Signaling >> Transcription >> Co-factors
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Other

Applications

Show applications key

Our Abpromise guarantee covers the use of ab45301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    IHC-P

     IHC-P: 1/100. Incubate a...Read more →

    IHC-P: 1/100. Incubate at 100°C for 10 min with citrate buffer pH6.0 before incubating with primary antibody.

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    IP

     IP: Use at an assay depe...Read more →

    IP: Use at an assay dependent dilution.

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    ChIP

     ChIP: Use at an assay de...Read more →

    ChIP: Use at an assay dependent dilution.

Target

Function

Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma.

Tissue specificity

Ubiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer (at protein level).

Sequence similarities

Belongs to the JARID1 histone demethylase family.
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 3 PHD-type zinc fingers.

Domain

Both the JmjC domain and the JmjN domain are required for enzymatic activity.
The 2 first PHD-type zinc finger domains are required for transcription repression activity.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ9UGL1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • JARID1A antibody
  • Cancer/testis antigen 31 antibody
  • CT31 antibody
  • Histone demethylase JARID1B antibody
  • JARID1B antibody
  • Jumonji AT rich interactive domain 1B antibody
  • Jumonji, AT rich interactive domain 1A antibody
  • Jumonji/ARID domain-containing protein 1B antibody
  • KDM5B antibody
  • KDM5B_HUMAN antibody
  • Lysine specific demethylase 5B antibody
  • Lysine-specific demethylase 5B antibody
  • PLU-1 antibody
  • PUT1 antibody
  • rbbp2 antibody
  • RBBP2H1A antibody
  • RBP2 H1 antibody
  • RBP2 like antibody
  • RBP2-H1 antibody
  • Retinoblastoma-binding protein 2 homolog 1 antibody
see all

Database links

References for Anti-KDM5B / PLU1 / Jarid1B antibody - ChIP Grade (ab45301)

This product has been referenced in:

  • Tao Y  et al. Treatment of breast cancer cells with DNA demethylating agents leads to a release of Pol II stalling at genes with DNA-hypermethylated regions upstream of TSS. Nucleic Acids Res : (2011). ChIP; Human.Read more (PubMed: 21880597) »
  • Gatta R & Mantovani R NF-Y substitutes H2A-H2B on active cell-cycle promoters: recruitment of CoREST-KDM1 and fine-tuning of H3 methylations. Nucleic Acids Res 36:6592-607 (2008). ChIP; Human.Read more (PubMed: 18940868) »

See all 2 publications for this product

Publishing research using ab45301? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"