Anti-KDM5B / PLU1 / Jarid1B antibody (ab186056)

Overview

  • Product name
    Anti-KDM5B / PLU1 / Jarid1B antibody
    See all KDM5B / PLU1 / Jarid1B primary antibodies
  • Description
    Rabbit polyclonal to KDM5B / PLU1 / Jarid1B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human KDM5B/ PLU1/ Jarid1B aa 1534-1544 (C terminal). NCBI Accession No. NP_006609.3
    Sequence:

    RCTVKDAPSRK


    Database link: Q9UGL1

  • Positive control
    • HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab186056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/80 - 1/100.
Flow Cyt 1/100.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma.
  • Tissue specificity
    Ubiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer (at protein level).
  • Sequence similarities
    Belongs to the JARID1 histone demethylase family.
    Contains 1 ARID domain.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
    Contains 3 PHD-type zinc fingers.
  • Domain
    Both the JmjC domain and the JmjN domain are required for enzymatic activity.
    The 2 first PHD-type zinc finger domains are required for transcription repression activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancer/testis antigen 31 antibody
    • CT31 antibody
    • Histone demethylase JARID1B antibody
    • JARID1B antibody
    • Jumonji AT rich interactive domain 1B antibody
    • Jumonji/ARID domain-containing protein 1B antibody
    • Kdm5b antibody
    • KDM5B_HUMAN antibody
    • Lysine (K) specific demethylase 5B antibody
    • Lysine demethylase 5B antibody
    • Lysine specific demethylase 5B antibody
    • Lysine-specific demethylase 5B antibody
    • PLU-1 antibody
    • PLU1 antibody
    • PPP1R98 antibody
    • Protein phosphatase 1 regulatory subunit 98 antibody
    • PUT1 antibody
    • putative DNA/chromatin binding motif antibody
    • RBBP2H1A antibody
    • RBP2 like antibody
    • RBP2-H1 antibody
    • Retinoblastoma-binding protein 2 homolog 1 antibody
    • Retinoblastoma-binding protein 2 homolog 1A antibody
    see all

Images

  • Immunofluorescence analysis of methanol-fixed HeLa cells, labeling KDM5B / PLU1 / Jarid1B with either A) Control without primary antibody, or B) ab186056 at 10 µg/ml. As secondary antibody goat anti-rabbit IgG FITC was used at a 1/200 dilution.

  • Flow cytometry analysis of fixed HeLa cells labeling KDM5B / PLU1 / Jarid1B using: 1) A rabbit IgG-FITC antibody at 0.01 µg/ml (red); 2) ab186056 at 5 µg/ml (yellow); 3) ab186056 at 10 µg/ml (blue). Immune complexes were detected with goat anti-rabbit IgG FITC at a 1/200 dilution.

References

ab186056 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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