The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/80 - 1/100.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma.
Ubiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer (at protein level).
Immunofluorescence analysis of methanol-fixed HeLa cells, labeling KDM5B / PLU1 / Jarid1B with either A) Control without primary antibody, or B) ab186056 at 10 µg/ml. As secondary antibody goat anti-rabbit IgG FITC was used at a 1/200 dilution.
Flow cytometry analysis of fixed HeLa cells labeling KDM5B / PLU1 / Jarid1B using: 1) A rabbit IgG-FITC antibody at 0.01 µg/ml (red); 2) ab186056 at 5 µg/ml (yellow); 3) ab186056 at 10 µg/ml (blue). Immune complexes were detected with goat anti-rabbit IgG FITC at a 1/200 dilution.