Anti-KDM5B / PLU1 / Jarid1B antibody (ab56759)

Overview

  • Product name
    Anti-KDM5B / PLU1 / Jarid1B antibody
    See all KDM5B / PLU1 / Jarid1B primary antibodies
  • Description
    Mouse monoclonal to KDM5B / PLU1 / Jarid1B
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: AKRMRAEAMN IKIEPEETTE ARTHNLRRRM GCPTPKCENE KEMKSSIKQE PIERKDYIVE NEKEKPKSRS KKATNAVDLY VCLLCGSGN, corresponding to amino acids 231-320 of Human PLU1/ Jarid1B

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab181089 as a replacement.

Properties

Applications

Our Abpromise guarantee covers the use of ab56759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 190 kDa.
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma.
  • Tissue specificity
    Ubiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer (at protein level).
  • Sequence similarities
    Belongs to the JARID1 histone demethylase family.
    Contains 1 ARID domain.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
    Contains 3 PHD-type zinc fingers.
  • Domain
    Both the JmjC domain and the JmjN domain are required for enzymatic activity.
    The 2 first PHD-type zinc finger domains are required for transcription repression activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancer/testis antigen 31 antibody
    • CT31 antibody
    • Histone demethylase JARID1B antibody
    • JARID1B antibody
    • Jumonji AT rich interactive domain 1B antibody
    • Jumonji/ARID domain-containing protein 1B antibody
    • Kdm5b antibody
    • KDM5B_HUMAN antibody
    • Lysine (K) specific demethylase 5B antibody
    • Lysine demethylase 5B antibody
    • Lysine specific demethylase 5B antibody
    • Lysine-specific demethylase 5B antibody
    • PLU-1 antibody
    • PLU1 antibody
    • PPP1R98 antibody
    • Protein phosphatase 1 regulatory subunit 98 antibody
    • PUT1 antibody
    • putative DNA/chromatin binding motif antibody
    • RBBP2H1A antibody
    • RBP2 like antibody
    • RBP2-H1 antibody
    • Retinoblastoma-binding protein 2 homolog 1 antibody
    • Retinoblastoma-binding protein 2 homolog 1A antibody
    see all

Images

  • PLU1 / Jarid1B antibody (ab56759) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human colon.


  • Predicted band size : 190 kDa
    PLU1 / Jarid1B antibody (ab56759) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • All lanes : Anti-KDM5B / PLU1 / Jarid1B antibody (ab56759) at 1/500 dilution

    Lane 1 : Human Pancreatic Cancer whole cell lysate (Line AsPc1)
    Lane 2 : Human Pancreatic Cancer whole cell lysate (line Panc1)
    Lane 3 : Human Pancreatic Cancer whole cell lysate (Line CaPan1)

    Lysates/proteins at 35 µg per lane.

    Secondary
    An HRP-conjugated Sheep anti-mouse polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 190 kDa
    Observed band size : 190 kDa


    Exposure time : 15 minutes

    This image is courtesy of an Abreview submitted by Pawel Mazur

    Blocking Step: 10% Milk for 1 hour at room temperature

    See Abreview

  • ab56759 staining KDM5B/PLU1/Jarid1B in Human pancreatic cancer tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with PBS + Triton 0.025% and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/100 in PBS) for 8 hours at 4°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab56759 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26759, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing MCF7 cells stained with ab56759 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56759, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:
  • Grandy RA  et al. Genome-Wide Studies Reveal that H3K4me3 Modification in Bivalent Genes Is Dynamically Regulated during the Pluripotent Cell Cycle and Stabilized upon Differentiation. Mol Cell Biol 36:615-27 (2015). WB ; Human . Read more (PubMed: 26644406) »
  • Kaushik G  et al. Honokiol inhibits melanoma stem cells by targeting notch signaling. Mol Carcinog 54:1710-21 (2015). Read more (PubMed: 25491779) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10% SDS)
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Apr 18 2014

Thank you for contacting us.

I am sorry to hear you have been experiencing problems with ab50598. I have contacted the lab to obtain images for bothab45301 and ab50958. Could you confirm whether ab56759 worked successfully? I would also like...

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Thank you for your enquiry regarding ab56759 and ab85454; and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

I would recommend the following posi...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human pancreatic cancer)
Specification
Human pancreatic cancer
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
Yes - PBS + Triton 0.025%
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jul 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (pancreatic cancer)
Loading amount
35 µg
Specification
pancreatic cancer
Gel Running Conditions
Non-reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Pawel Mazur

Verified customer

Submitted Jun 09 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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