Anti-KDM5B / PLU1 / Jarid1B antibody (ab84883)
Key features and details
- Rabbit polyclonal to KDM5B / PLU1 / Jarid1B
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KDM5B / PLU1 / Jarid1B antibody
See all KDM5B / PLU1 / Jarid1B primary antibodies -
Description
Rabbit polyclonal to KDM5B / PLU1 / Jarid1B -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
Immunogen
Synthetic peptide corresponding to a region between residue 1494 and 1544 of human KDM5B/ PLU1/ Jarid1B (accession number: NP_006609.3)
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Positive control
- Human Controls: Breast Carcinoma, Prostate Carcinoma
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified using an epitope specific to KDM5B / PLU1 / Jarid1B immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Notes |
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Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma. -
Tissue specificity
Ubiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer (at protein level). -
Sequence similarities
Belongs to the JARID1 histone demethylase family.
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 3 PHD-type zinc fingers. -
Domain
Both the JmjC domain and the JmjN domain are required for enzymatic activity.
The 2 first PHD-type zinc finger domains are required for transcription repression activity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10765 Human
- Omim: 605393 Human
- SwissProt: Q9UGL1 Human
- Unigene: 443650 Human
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Alternative names
- Cancer/testis antigen 31 antibody
- CT31 antibody
- Histone demethylase JARID1B antibody
see all
Images
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Detection of Human Jarid1B in a formalin-fixed paraffin-embedded section of human prostate carcinoma by Immunohistochemistry using ab84883 at a dilution of 1:500. Detection: DAB staining.
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Detection of Human Jarid1B in a formalin-fixed paraffin-embedded section of human breast carcinoma by Immunohistochemistry using ab84883 at a dilution of 1:250. Detection: DAB staining.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab84883 has not yet been referenced specifically in any publications.