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Products:Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Anti-KDM5C / Jarid1C / SMCX antibody - ChIP Grade (ab34718)
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ab35501
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab34718? Please let us know so that we can cite the reference in this datasheet
ab34718 has been referenced in 2 publications.
- Huang PH et al. Histone deacetylase inhibitors stimulate histone H3 lysine 4 methylation in part via transcriptional repression of histone H3 lysine 4 demethylases. Mol Pharmacol 79:197-206 (2011). WB, ChIP; Human. PubMed: 20959362
- Kim JH & Youn HD C-terminal binding protein maintains mitochondrial activities. Cell Death Differ 16:584-92 (2009). ChIP; Human. PubMed: 19136938
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - Jarid1C / SMCX antibody (ab34718)
Anti-KDM5C / Jarid1C / SMCX antibody - ChIP Grade (ab34718) at 1/250 dilution +
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 176 kDa
Observed band size : 176 kDa
Immunocytochemistry/ Immunofluorescence - Anti-KDM5C / Jarid1C / SMCX antibody - ChIP Grade (ab34718)
ICC/IF image of ab34718 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab34718 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
KDM5C / Jarid1C / SMCX antibody for WB in Human (34718)
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