Products:Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
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ab35501 |
If your product does not perform as described on this datasheet, we will refund or replace your product...
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I entered information for an abreview for this product last month. For some reason the review shows up as blank on the site even though I filled out the form, and I can't submit a new one because I already submitted the earlier one. I assume I can still use the code for a free antibody since a review was submitted, but I would like to submit one with the actual info and a picture if possible to put the information out there. Let me know if there's a way to do that please. Sorry about that, I'm not sure why that happened. |
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ANSWER: |
Thank you for contacting us. |
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We have successfully used the antibody in mouse samples. I would be happy to send some pictures-will that allow us to then use the code? Also, we would like to validate the antibody, so I'm wondering if the antigen is available for purchase. |
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ANSWER: |
Thank you for your email. |
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I am detecting a band at ˜80kDa instead of 176kDa. Is it possible to try another aliquot of antibody? |
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ANSWER: |
Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-KDM5C / Jarid1C / SMCX antibody - ChIP Grade (ab34718) at 1/250 dilution +
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 176 kDa
Observed band size : 176 kDa
ICC/IF image of ab34718 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab34718 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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