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Product name
Anti-KDM5C / Jarid1C / SMCX antibody - ChIP Grade
See all KDM5C / Jarid1C / SMCX products (4) ...
Description
Rabbit polyclonal to KDM5C / Jarid1C / SMCX - ChIP Grade
Tested applications
ChIP, WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Pig
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1500 to the C-terminus of Human Jarid1C / SMCX.
(Peptide available as ab355 01.)
Positive control
HEK 293 (Human embryonic kidney cell line) Whole Cell Lysate. This antibody gave a positive signal in the following Methanol fixed cell lines:HeLa, Hek293, HepG2, and MCF-7. This antibody gave a positive signal in the following Formaldehyde fixed cell lines:HepG2, and MCF-7.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
Epigenetics and Nuclear Signaling >> Transcription >> Co-factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab34718 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP: Use at an assay dependent dilution.
WB: 1/250. Detects a band of approximately 176 kDa (predicted molecular weight: 176 kDa).
ICC/IF: Use a concentration of 1 µg/ml.
Target
Function
Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements.
Tissue specificity
Expressed in all tissues examined. Highest levels found in brain and skeletal muscle.
Involvement in disease
Defects in KDM5C are the cause of mental retardation syndromic X-linked JARID1C-related (MRXSJ) [MIM:300534]. MRXSJ is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRXSJ patients manifest mental retardation associated with variable features such as slowly progressive spastic paraplegia, seizures, facial dysmorphism.
Sequence similarities
Belongs to the JARID1 histone demethylase family.
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 2 PHD-type zinc fingers.
Domain
The first PHD-type zinc finger domain recognizes and binds H3-K9Me3.
Both the JmjC domain and the JmjN domain are required for enzymatic activity.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP41229
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- SmcX protein antibody
- Xe169 protein antibody
- DXS1272E antibody
see all
Database links
- Entrez Gene: 8242 Human
- Entrez Gene: 100037295 Pig
- Omim: 314690 Human
- SwissProt: Q86WW4 Human
- SwissProt: Q7Z5S5 Human
- SwissProt: P41229 Human
- Unigene: 631768 Human
Anti-KDM5C / Jarid1C / SMCX antibody - ChIP Grade images:
Western blot - Jarid1C / SMCX antibody (ab34718)
Anti-KDM5C / Jarid1C / SMCX antibody - ChIP Grade (ab34718) at 1/250 dilution +
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 176 kDa
Observed band size : 176 kDa
Immunocytochemistry/ Immunofluorescence - Anti-KDM5C / Jarid1C / SMCX antibody - ChIP Grade (ab34718)
ICC/IF image of ab34718 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab34718 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-KDM5C / Jarid1C / SMCX antibody - ChIP Grade (ab34718)
This product has been referenced in:
- Huang PH et al. Histone deacetylase inhibitors stimulate histone H3 lysine 4 methylation in part via transcriptional repression of histone H3 lysine 4 demethylases. Mol Pharmacol 79:197-206 (2011). WB, ChIP; Human.Read more (PubMed: 20959362) »
- Kim JH & Youn HD C-terminal binding protein maintains mitochondrial activities. Cell Death Differ 16:584-92 (2009). ChIP; Human.Read more (PubMed: 19136938) »
See all 2 publications for this product
Publishing research using ab34718? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"