Anti-KDM5C / Jarid1C / SMCX antibody [2E4-E1-G8] (ab106068)

Overview

  • Product nameAnti-KDM5C / Jarid1C / SMCX antibody [2E4-E1-G8]
    See all KDM5C / Jarid1C / SMCX primary antibodies
  • Description
    Mouse monoclonal [2E4-E1-G8] to KDM5C / Jarid1C / SMCX
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human KDM5C/ Jarid1C/ SMCX (C terminal). Expressed in E. coli.
    Database link: P41229

  • Positive control
    • 293 whole cell lysate and CEM whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab106068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/100 - 1/2000. Predicted molecular weight: 176 kDa.
ELISA 1/5000 - 1/20000.

Target

  • FunctionHistone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements.
  • Tissue specificityExpressed in all tissues examined. Highest levels found in brain and skeletal muscle.
  • Involvement in diseaseDefects in KDM5C are the cause of mental retardation syndromic X-linked JARID1C-related (MRXSJ) [MIM:300534]. MRXSJ is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRXSJ patients manifest mental retardation associated with variable features such as slowly progressive spastic paraplegia, seizures, facial dysmorphism.
  • Sequence similaritiesBelongs to the JARID1 histone demethylase family.
    Contains 1 ARID domain.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
    Contains 2 PHD-type zinc fingers.
  • DomainThe first PHD-type zinc finger domain recognizes and binds H3-K9Me3.
    Both the JmjC domain and the JmjN domain are required for enzymatic activity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DXS1272E antibody
    • Histone demethylase JARID1C antibody
    • JARID1C antibody
    • JmjC domain containing protein SMCX antibody
    • Jumonji AT rich interactive domain 1C antibody
    • Jumonji, AT rich interactive domain 1C (RBP2 like) antibody
    • Jumonji/ARID domain-containing protein 1C antibody
    • KDM5C antibody
    • KDM5C_HUMAN antibody
    • Lysine (K) specific demethylase 5C antibody
    • Lysine-specific demethylase 5C antibody
    • MRX13 antibody
    • MRXJ antibody
    • MRXSCJ antibody
    • MRXSJ antibody
    • Protein SmcX antibody
    • Protein Xe169 antibody
    • rbp2 like protein antibody
    • Selected cDNA on X antibody
    • SMCX antibody
    • Smcx homolog X chromosome antibody
    • SmcX protein antibody
    • Smcy homolog X linked antibody
    • XE169 antibody
    • Xe169 protein antibody
    see all

Anti-KDM5C / Jarid1C / SMCX antibody [2E4-E1-G8] images

  • Immunofluorescence image of HeLa cells using an anti-KDM5C antibody (ab106068) at a dilution of 1:150.

  • All lanes : Anti-KDM5C / Jarid1C / SMCX antibody [2E4-E1-G8] (ab106068) at 1/1000 dilution

    Lane 1 : 293 whole cell lysate
    Lane 2 : CEM whole cell lysate

    Lysates/proteins at 40 µg per lane.


    Predicted band size : 176 kDa
    Observed band size : 176 kDa

References for Anti-KDM5C / Jarid1C / SMCX antibody [2E4-E1-G8] (ab106068)

ab106068 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"