The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Predicted molecular weight: 175 kDa.
Use at an assay dependent concentration.
Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements.
Expressed in all tissues examined. Highest levels found in brain and skeletal muscle.
Involvement in disease
Defects in KDM5C are the cause of mental retardation syndromic X-linked JARID1C-related (MRXSJ) [MIM:300534]. MRXSJ is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRXSJ patients manifest mental retardation associated with variable features such as slowly progressive spastic paraplegia, seizures, facial dysmorphism.
Jumonji, AT rich interactive domain 1C (RBP2 like) antibody
Jumonji/ARID domain-containing protein 1C antibody
Lysine (K) specific demethylase 5C antibody
Lysine-specific demethylase 5C antibody
Protein SmcX antibody
Protein Xe169 antibody
rbp2 like protein antibody
Selected cDNA on X antibody
Smcx homolog X chromosome antibody
SmcX protein antibody
Smcy homolog X linked antibody
Xe169 protein antibody
Western blot - Anti-KDM5C / Jarid1C / SMCX antibody (ab190180)
All lanes : Anti-KDM5C / Jarid1C / SMCX antibody (ab190180) at 1/2000 dilution
Lane 1 : Human U2OS cell lysate Lane 2 : Human U2OS cells transfected with KDM5C / Jarid1C / SMCX siRNA Lane 3 : Mouse embryo fibroblasts (MEFs) Lane 4 : Rat INS1 cell lysate Lane 5 : Chicken DT40 cell lysate