Anti-KDM5C / Jarid1C / SMCX antibody - ChIP Grade (ab34718)

Overview

  • Product nameAnti-KDM5C / Jarid1C / SMCX antibody - ChIP Grade
    See all KDM5C / Jarid1C / SMCX primary antibodies
  • Description
    Rabbit polyclonal to KDM5C / Jarid1C / SMCX - ChIP Grade
  • Tested applicationsSuitable for: ChIP, IHC-FoFr, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1500 to the C-terminus of Human Jarid1C/ SMCX.

    (Peptide available as ab35501.)

  • Positive control
    • This antibody gave a positive signal in HEK 293 (Human embryonic kidney cell line), and Y79 (Human retinoblastoma cell line) Whole Cell Lysates. This antibody gave a positive signal in the following Methanol fixed cell lines:HeLa, Hek293, HepG2, and MCF-7. This antibody gave a positive signal in the following Formaldehyde fixed cell lines:HepG2, and MCF-7.

Properties

Applications

Our Abpromise guarantee covers the use of ab34718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.
WB 1/250. Detects a band of approximately 180 kDa (predicted molecular weight: 176 kDa).

Abcam recommends using milk as the blocking agent.

ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionHistone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements.
  • Tissue specificityExpressed in all tissues examined. Highest levels found in brain and skeletal muscle.
  • Involvement in diseaseDefects in KDM5C are the cause of mental retardation syndromic X-linked JARID1C-related (MRXSJ) [MIM:300534]. MRXSJ is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRXSJ patients manifest mental retardation associated with variable features such as slowly progressive spastic paraplegia, seizures, facial dysmorphism.
  • Sequence similaritiesBelongs to the JARID1 histone demethylase family.
    Contains 1 ARID domain.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
    Contains 2 PHD-type zinc fingers.
  • DomainThe first PHD-type zinc finger domain recognizes and binds H3-K9Me3.
    Both the JmjC domain and the JmjN domain are required for enzymatic activity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DXS1272E antibody
    • Histone demethylase JARID1C antibody
    • JARID1C antibody
    • JmjC domain containing protein SMCX antibody
    • Jumonji AT rich interactive domain 1C antibody
    • Jumonji, AT rich interactive domain 1C (RBP2 like) antibody
    • Jumonji/ARID domain-containing protein 1C antibody
    • KDM5C antibody
    • KDM5C_HUMAN antibody
    • Lysine (K) specific demethylase 5C antibody
    • Lysine-specific demethylase 5C antibody
    • MRX13 antibody
    • MRXJ antibody
    • MRXSCJ antibody
    • MRXSJ antibody
    • Protein SmcX antibody
    • Protein Xe169 antibody
    • rbp2 like protein antibody
    • Selected cDNA on X antibody
    • SMCX antibody
    • Smcx homolog X chromosome antibody
    • SmcX protein antibody
    • Smcy homolog X linked antibody
    • XE169 antibody
    • Xe169 protein antibody
    see all

Anti-KDM5C / Jarid1C / SMCX antibody - ChIP Grade images

  • All lanes : Anti-KDM5C / Jarid1C / SMCX antibody - ChIP Grade (ab34718) at 1 µg/ml

    Lane 1 : HEK293 (Human) Whole Cell Lysate
    Lane 2 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 176 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa,58 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab34718 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • Anti-KDM5C / Jarid1C / SMCX antibody - ChIP Grade (ab34718) at 1/250 dilution + HEK293 whole cell lysate (ab7902) at 20 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 176 kDa
    Observed band size : 176 kDa
  • ICC/IF image of ab34718 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab34718 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-KDM5C / Jarid1C / SMCX antibody - ChIP Grade (ab34718)

This product has been referenced in:
  • Fujita T  et al. Identification of telomere-associated molecules by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP). Sci Rep 3:3171 (2013). ICC/IF ; Human . Read more (PubMed: 24201379) »
  • Huang PH  et al. Histone deacetylase inhibitors stimulate histone H3 lysine 4 methylation in part via transcriptional repression of histone H3 lysine 4 demethylases. Mol Pharmacol 79:197-206 (2011). WB, ChIP ; Human . Read more (PubMed: 20959362) »

See all 3 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - 0.5% Triton-X100 in PBS
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

Submitted Feb 27 2015

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (7,5% gel)
Sample Human Cell lysate - whole cell (different cell lines)
Specification different cell lines
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Nov 29 2013

Thank you for contacting us.

I have checked the homology between KDM5D and KDM5C, and for the immunogen region of ab34718, the homology is poor and the antibody would not cross react.

Unfortunately, we do not carry antibodies that ...

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Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (brain-perfused frozen sections)
Specification brain-perfused frozen sections
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 30 2012

Thank you for contacting us.

The abreview (xxx) is in dormant status as it was not edited for over 28 days. I have just put it back into editing status and you should be able to see it. Lots of information is still missing which would need t...

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Thank you for your email.
I am glad to hear that the antibody has worked successfully in the untested species.

Here iswhat you need to do in order to activate your discount code:


The code will give you 1 free PRIMARY ANTI...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Y79 retinoblastoma cell line)
Loading amount 10 µg
Specification Y79 retinoblastoma cell line
Gel Running Conditions Reduced Denaturing (10%)
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Sep 12 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"