KDM5/JARID Activity Quantification Assay Kit (Colorimetric) (ab113463)

Overview

  • Product name
    KDM5/JARID Activity Quantification Assay Kit (Colorimetric)
    See all KDM5/JARID kits
  • Assay type
    Enzyme activity
  • Assay time
    3h 00m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Mammal
  • Product overview

    KDM5/JARID (Lysine-specific demethylase 5) is a family of histone demethyltransferases involved in H3(meK4) specific demethylation. KDM5/JARID demethylases are JmjC proteins that remove di- and tri-methylation from H3(meK4) but have no activity on mono-methylated residues.

    KDM5/JARID Activity Quantification Assay Kit (Colorimetric) (ab113463) is designed for the easy and fast colorimetric measurement of KDM5/JARID activity or inhibition. The antibody-based, immunospecific method directly detects KDM5/JARID-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab113463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • Demonstration of high sensitivity of the KDM5/JARID activity assay achieved by using A549 nuclear extracts with ab113463.

Protocols

References

ab113463 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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