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KDM5/JARID (Lysine-specific demethylase 5) is a family of histone demethyltransferases involved in H3(meK4) specific demethylation. KDM5/JARID demethylases are JmjC proteins that remove di- and tri-methylation from H3(meK4) but have no activity on mono-methylated residues.
KDM5/JARID Activity Quantification Assay Kit (Colorimetric) (ab113463) is designed for the easy and fast colorimetric measurement of KDM5/JARID activity or inhibition. The antibody-based, immunospecific method directly detects KDM5/JARID-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.
|Components||48 tests||96 tests|
|10X Wash Buffer||1 x 14ml||1 x 28ml|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|Adhesive Covering Film||1 unit||1 unit|
|Capture Antibody, 1000 µg/mL||1 x 5µl||1 x 10µl|
|Co-factor 1||1 x 30µl||1 x 60µl|
|Co-factor 2||1 x 30µl||1 x 60µl|
|Co-factor 3||1 x 30µl||1 x 60µl|
|Detection Antibody, 400 µg/mL||1 x 6µl||1 x 12µl|
|Developer Solution||1 x 5ml||1 x 10ml|
|JARID Assay Buffer||1 x 4ml||1 x 8ml|
|JARID Assay Standard, 50 µg/mL||1 x 10µl||1 x 20µl|
|JARID Substrate, 50 µg/mL||1 x 60µl||1 x 120µl|
|Stop Solution||1 x 5ml||1 x 10ml|
Our Abpromise guarantee covers the use of ab113463 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab113463 has not yet been referenced specifically in any publications.