KDM5/JARID Activity Quantification Assay Kit (Fluorometric) (ab113464)

Overview

  • Product nameKDM5/JARID Activity Quantification Assay Kit (Fluorometric)
    See all KDM5/JARID kits
  • Assay time
    3h 00m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    KDM5/JARID (Lysine-specific demethylase 5) is a family of histone demethyltransferases involved in H3(meK4) specific demethylation. KDM5/JARID demethylases are JmjC proteins that remove di- and tri-methylation from H3(meK4) but have no activity on mono-methylated residues.

    KDM5/JARID Activity Quantification Assay Kit (Fluorometric) (ab113464) is designed for the easy and fast fluorometric measurement of KDM5/JARID activity or inhibition. The antibody-based, immunospecific method directly detects KDM5/JARID-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.

  • Tested applicationsFunctional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab113464 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    KDM5/JARID Activity Quantification Assay Kit (Fluorometric) images

    • Demonstration of high sensitivity of KDM5/JARID activity assay achieved by using A549 nuclear extracts with ab113464

    Protocols

    References for KDM5/JARID Activity Quantification Assay Kit (Fluorometric) (ab113464)

    ab113464 has not yet been referenced specifically in any publications.

    Product Wall

    Ce kit est pour mesurer l’activité de KDM5 qui est une enzyme qui enlève les methyl des protéines et surtout des histones.

    KDM5 a effectivement comme substrats, H3K4me3 et H3K4me2 et produit H3K4 mono-méthyl&e...

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    Thank you for your interest in ab113455 & ab113464.

    You are correct that both these kits measure demethylase activity. The major difference between these 2 kits is the substrate captured on the well. For ab113455, the substrate is a dimet...

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    Thank you for your call last week and for your patience while I have been in touch with my colleagues in the lab regarding ab113464.

    The substrate is a short peptide, and the detection antibody binds to mono-methylated histone.

    I ho...

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    Thank you for contacting us. Tri-methylated histone H3-K4 is a substrate for JARID enzyme which removes methyl group from substrate, which is then detected by antibodies present in the kit. So the kit antibodies will detect the JARID de-methyla...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"