KDM5/JARID Activity Quantification Assay Kit (Fluorometric) (ab113464)

Overview

  • Product name
    KDM5/JARID Activity Quantification Assay Kit (Fluorometric)
    See all KDM5/JARID kits
  • Assay type
    Enzyme activity
  • Assay time
    3h 00m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Mammal
  • Product overview

    KDM5/JARID (Lysine-specific demethylase 5) is a family of histone demethyltransferases involved in H3(meK4) specific demethylation. KDM5/JARID demethylases are JmjC proteins that remove di- and tri-methylation from H3(meK4) but have no activity on mono-methylated residues.

    KDM5/JARID Activity Quantification Assay Kit (Fluorometric) (ab113464) is designed for the easy and fast fluorometric measurement of KDM5/JARID activity or inhibition. The antibody-based, immunospecific method directly detects KDM5/JARID-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab113464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • Demonstration of high sensitivity of KDM5/JARID activity assay achieved by using A549 nuclear extracts with ab113464

Protocols

References

ab113464 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Ce kit est pour mesurer l’activité de KDM5 qui est une enzyme qui enlève les methyl des protéines et surtout des histones.

KDM5 a effectivement comme substrats, H3K4me3 et H3K4me2 et produit H3K4 mono-méthyl&e...

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Thank you for your interest in ab113455 & ab113464.

You are correct that both these kits measure demethylase activity. The major difference between these 2 kits is the substrate captured on the well. For ab113455, the substrate is a dimet...

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Thank you for your call last week and for your patience while I have been in touch with my colleagues in the lab regarding ab113464.

The substrate is a short peptide, and the detection antibody binds to mono-methylated histone.

I ho...

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Thank you for contacting us. Tri-methylated histone H3-K4 is a substrate for JARID enzyme which removes methyl group from substrate, which is then detected by antibodies present in the kit. So the kit antibodies will detect the JARID de-methyla...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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