Our Abpromise guarantee covers the use of ab38113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC (PFA fixed)||Use a concentration of 5 µg/ml.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|ChIP||Use at an assay dependent concentration. PubMed: 23932971|
Chromatin was prepared from mouse hippocampus. Crosslinking (X-ChIP) was performed for 15 minutes with 1% formaldehyde. ChIP was performed with ab38113 at 1/50 dilution (black) or normal rabbit IgG (white). The positive control was Beta-actin. The negative control was HoxA1 (a polycomb repressed gene in hippocampus).