Overview

  • Product nameAnti-KDM6B / JMJD3 antibody
    See all KDM6B / JMJD3 primary antibodies
  • Description
    Rabbit polyclonal to KDM6B / JMJD3
  • Tested applicationsSuitable for: IHC (PFA fixed), ICC/IF, ChIPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human KDM6B/ JMJD3 aa 1-100 conjugated to keyhole limpet haemocyanin.
    Database link: O15054
    (Peptide available as ab38112)

Properties

Applications

Our Abpromise guarantee covers the use of ab38113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC (PFA fixed) Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
ChIP Use at an assay dependent concentration. PubMed: 23932971

Target

  • FunctionHistone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation.
  • Sequence similaritiesBelongs to the UTX family.
    Contains 1 JmjC domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Histone demethylase JMJD3 antibody
    • JmjC domain containing protein 3 antibody
    • JmjC domain-containing protein 3 antibody
    • Jumonji D3 antibody
    • Jumonji domain containing 3 antibody
    • Jumonji domain containing 3 histone lysine demethylase antibody
    • Jumonji domain containing protein 3 antibody
    • Jumonji domain-containing protein 3 antibody
    • Kdm6b antibody
    • KDM6B_HUMAN antibody
    • KIAA0346 antibody
    • Lysine demethylase 6B antibody
    • Lysine K specific demethylase 6B antibody
    • Lysine specific demethylase 6B antibody
    • Lysine-specific demethylase 6B antibody
    see all

Anti-KDM6B / JMJD3 antibody images

  • ICC/IF image of ab38113 stained HeLa cells. The cells were 100% methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab38113, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, MCF7 and HepG2 cells fixed in 100% methanol at 5ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
  • IHC image of KDM6B/JMJD3 staining in human liver carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab38113, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References for Anti-KDM6B / JMJD3 antibody (ab38113)

This product has been referenced in:
  • Henriquez B  et al. Ezh1 and Ezh2 differentially regulate PSD-95 gene transcription in developing hippocampal neurons. Mol Cell Neurosci 57:130-43 (2013). ChIP ; Rat . Read more (PubMed: 23932971) »
  • Gomez-Sanchez JA  et al. Epigenetic induction of the Ink4a/Arf locus prevents Schwann cell overproliferation during nerve regeneration and after tumorigenic challenge. Brain 136:2262-78 (2013). IHC-Fr ; Mouse, Human . Read more (PubMed: 23748155) »

See all 7 Publications for this product

Product Wall

Application ChIP
Detection step Real-time PCR
Sample Mouse Tissue lysate - nuclear (Hippocampus)
Specification Hippocampus
Negative control HoxA1, a Polycomb repressed gene in hippocampus, and normal Rabbit IgG
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehide 1%
Positive control b-actin
Username

Dr. Ernest Palomer

Verified customer

Submitted Nov 11 2014

Application Western blot
Loading amount 5 µg
Gel Running Conditions Reduced Denaturing
Sample Chicken Tissue lysate - whole (Brain)
Specification Brain
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Ms. Ying Jiang

Verified customer

Submitted Aug 07 2013

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guar...

Read More

Thank you for your reply and for confirming the steps of your protocol.


Since these antibodies are Fast Track antibodies, they are only guaranteed to work in ELISA using the peptide immunogen. These are not tested in-house, and therefor...

Read More
Application Western blot
Sample Human Cell lysate - whole cell (HeLa, HEK293, Jurkat, HL-60)
Loading amount 20 µg
Specification HeLa, HEK293, Jurkat, HL-60
Gel Running Conditions Reduced Denaturing (Pierce precise protein gel 4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Feb 22 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100 in PBS
Username

Dr. Kirk McManus

Verified customer

Submitted Mar 16 2009

Application Western blot
Sample Human Cell lysate - whole cell (keratinocyte)
Loading amount 100 µg
Specification keratinocyte
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 18 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"