Anti-KDM6B / JMJD3 antibody (ab84200)

Overview

  • Product nameAnti-KDM6B / JMJD3 antibody
    See all KDM6B / JMJD3 primary antibodies
  • Description
    Rabbit polyclonal to KDM6B / JMJD3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to Human KDM6B/ JMJD3 aa 2-51 (N terminal).
    Sequence:

    HRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASI


    Database link: O15054

  • Positive control
    • Human fetal lung lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 181 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionHistone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation.
  • Sequence similaritiesBelongs to the UTX family.
    Contains 1 JmjC domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Histone demethylase JMJD3 antibody
    • JmjC domain containing protein 3 antibody
    • JmjC domain-containing protein 3 antibody
    • Jumonji D3 antibody
    • Jumonji domain containing 3 antibody
    • Jumonji domain containing 3 histone lysine demethylase antibody
    • Jumonji domain containing protein 3 antibody
    • Jumonji domain-containing protein 3 antibody
    • Kdm6b antibody
    • KDM6B_HUMAN antibody
    • KIAA0346 antibody
    • Lysine demethylase 6B antibody
    • Lysine K specific demethylase 6B antibody
    • Lysine specific demethylase 6B antibody
    • Lysine-specific demethylase 6B antibody
    see all

Anti-KDM6B / JMJD3 antibody images

  • Anti-KDM6B / JMJD3 antibody (ab84200) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 181 kDa
    Observed band size : >168 kDa (why is the actual band size different from the predicted?)

References for Anti-KDM6B / JMJD3 antibody (ab84200)

ab84200 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (5-15%)
Sample Mouse Cell lysate - whole cell (Human Fibroblasts Mouse Primary hippocampal neuron)
Specification Human Fibroblasts Mouse Primary hippocampal neuron
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Verified customer

Submitted Sep 27 2013

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guar...

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Thank you for your reply.
I am sorry to hear that you are struggeling so much with these antibodies.

I have checked again the datasheet for ab85392: Mouse was shown to work in the publication that is listed on the datasheet:

Mi...

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Thank you for your participation in Abcam's post complaint survey.

I am sorry to hear that the KDM6B antibodies do not work for you satisfactorily.

According to our correspondence, your are working on mouse samples and only ab85392 ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"