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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> HOX
Overview
Product name
Anti-KDM6B / JMJD3 antibody - ChIP Grade
See all KDM6B / JMJD3 products (3) ...
Description
Rabbit polyclonal to KDM6B / JMJD3 - ChIP Grade
Tested applications
ChIP, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide, corresponding to a region within amino acids 600-650 of Mouse KDM6B / JMJD3 [Swiss-Prot# P22079]
Positive control
HepG2 cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 30% Glycerol, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
-
Western blot - KDM6B / JMJD3 antibody (ab85392)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab85392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP: Use at an assay dependent dilution. (PubMed: 21095589)
WB: Use a concentration of 2 µg/mlPredicted molecular weight: 180 kDa.
Target
Function
Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation.
Sequence similarities
Belongs to the UTX family.
Contains 1 JmjC domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO15054
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Histone demethylase JMJD3 antibody
- JmjC domain containing protein 3 antibody
- JmjC domain-containing protein 3 antibody
see all
Database links
- Entrez Gene: 23135 Human
- Entrez Gene: 216850 Mouse
- Omim: 611577 Human
- SwissProt: O15054 Human
- SwissProt: Q5NCY0 Mouse
- Unigene: 223678 Human
- Unigene: 261201 Mouse
- Unigene: 472522 Mouse
Anti-KDM6B / JMJD3 antibody - ChIP Grade images:
Western blot - KDM6B / JMJD3 antibody (ab85392)
Anti-KDM6B / JMJD3 antibody - ChIP Grade (ab85392) at 2 µg/ml + HepG2 cell lysate
Predicted band size : 180 kDa
Observed band size : 180 kDa
Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.
References for Anti-KDM6B / JMJD3 antibody - ChIP Grade (ab85392)
This product has been referenced in:
- Miller SAet al. Jmjd3 and UTX Play a Demethylase-Independent Role in Chromatin Remodeling to Regulate T-Box Family Member-Dependent Gene Expression. Mol Cell 40:594-605 (2010). WB, ChIP; Mouse.Read more (PubMed: 21095589) »
See 1 publication for this product
Publishing research using ab85392? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"