Recombinant
RabMAb

Anti-Keratin 12 antibody [EPR1609(2)] (ab124975)

Overview

  • Product name
    Anti-Keratin 12 antibody [EPR1609(2)]
    See all Keratin 12 primary antibodies
  • Description
    Rabbit monoclonal [EPR1609(2)] to Keratin 12
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C-terminus of Human Keratin 12 protein (Q99456).

  • Positive control
    • Rat eyeball lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab124975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      May play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity.
    • Tissue specificity
      Cornea specific.
    • Involvement in disease
      Defects in KRT12 are a cause of Meesmann corneal dystrophy (MECD) [MIM:122100]; also abbreviated MCD and known as juvenile epithelial corneal dystrophy of Meesmann. MECD is an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts.
    • Sequence similarities
      Belongs to the intermediate filament family.
    • Information by UniProt
    • Database links
    • Alternative names
      • CK-12 antibody
      • Cytokeratin-12 antibody
      • K12 antibody
      • K1C12_HUMAN antibody
      • Keratin 12 antibody
      • Keratin antibody
      • Keratin, type I cytoskeletal 12 antibody
      • Keratin-12 antibody
      • KRT12 antibody
      • type I cytoskeletal 12 antibody
      see all

    Images

    • Anti-Keratin 12 antibody [EPR1609(2)] (ab124975) at 1/1000 dilution + Rat eyeball lysate at 10 µg

      Predicted band size : 54 kDa

    References

    This product has been referenced in:
    • Li G  et al. Transcription Factor PAX6 (Paired Box 6) Controls Limbal Stem Cell Lineage in Development and Disease. J Biol Chem 290:20448-54 (2015). Read more (PubMed: 26045558) »
    • Ouyang H  et al. WNT7A and PAX6 define corneal epithelium homeostasis and pathogenesis. Nature 511:358-61 (2014). Read more (PubMed: 25030175) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Thank you for your inquiry and your interest in our products.

    I searched our catalogue and we currently do not have a cytokeratin 3 antibody that is directly conjugated. We do carry ab77869, which has been tested in FC with rabbit, cow and hu...

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