Overview

  • Product name
  • Description
    Rabbit polyclonal to Keratin 77
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Keratin 77 .

  • Positive control
    • This antibody gave a positive signal in both Human Skin and Placenta tissue lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 62 kDa).

Target

  • Tissue specificity
    Expressed exclusively in skin.
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Post-translational
    modifications
    Undergoes deimination of some arginine residues (citrullination).
  • Information by UniProt
  • Database links
  • Alternative names
    • CK-1B antibody
    • Cytokeratin-1B antibody
    • K1B antibody
    • K2C1B_HUMAN antibody
    • K77 antibody
    • KB39 antibody
    • Keratin 1B antibody
    • Keratin, type II cytoskeletal 1b antibody
    • Keratin-77 antibody
    • KRT1B antibody
    • Krt77 antibody
    • Type-II keratin Kb39 antibody
    see all

Images

  • All lanes : Anti-Keratin 77 antibody (ab106847) at 1 µg/ml

    Lane 1 : Human skin tissue lysate - total protein (ab30166)
    Lane 2 : Human placenta tissue lysate - total protein (ab29745)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 62 kDa


    Exposure time : 2 minutes

References

ab106847 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab106847.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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