Anti-Keratin 77 antibody (ab106847)
Key features and details
- Rabbit polyclonal to Keratin 77
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Keratin 77 antibody
See all Keratin 77 primary antibodies -
Description
Rabbit polyclonal to Keratin 77 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Gorilla -
Immunogen
Synthetic peptide corresponding to Human Keratin 77 aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available asab126633) -
Positive control
- This antibody gave a positive signal in both Human Skin and Placenta tissue lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 62 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 62 kDa). |
Target
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Tissue specificity
Expressed exclusively in skin. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsUndergoes deimination of some arginine residues (citrullination). - Information by UniProt
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Database links
- Entrez Gene: 374454 Human
- Omim: 611158 Human
- SwissProt: Q7Z794 Human
- Unigene: 334989 Human
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Alternative names
- CK-1B antibody
- Cytokeratin-1B antibody
- K1B antibody
see all
Images
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All lanes : Anti-Keratin 77 antibody (ab106847) at 1 µg/ml
Lane 1 : Human skin tissue lysate - total protein (ab30166)
Lane 2 : Human placenta tissue lysate - total protein (ab29745)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 62 kDa
Exposure time: 2 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab106847 has not yet been referenced specifically in any publications.