Overview

  • Product name
    Anti-ketohexokinase antibody
    See all ketohexokinase primary antibodies
  • Description
    Rabbit polyclonal to ketohexokinase
  • Tested applications
    Suitable for: IHC-P, ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH selected within the last 50 aa of human ketohexokinase.

  • Positive control
    • Mouse kidney tissue lysate. IHC-P: FFPE human kidney tissue sections. IF/ICC: HeLa cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab38281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/1000.
WB 1/100 - 1/500. Predicted molecular weight: 33 kDa.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Tissue specificity
    Most abundant in liver, kidney, gut, spleen and pancreas. Low levels also found in adrenal, muscle, brain and eye.
  • Pathway
    Carbohydrate metabolism; fructose metabolism.
  • Involvement in disease
    Defects in KHK are the cause of fructosuria (FRUCT) [MIM:229800]. Fructosuria is a benign defect of intermediary metabolism.
  • Sequence similarities
    Belongs to the carbohydrate kinase pfkB family.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 2.7.1.3 antibody
    • Fructokinase antibody
    • Hepatic fructokinase antibody
    • Ketohexokinase antibody
    • Ketohexokinase isoform a antibody
    • KHK antibody
    • KHK_HUMAN antibody
    see all

Images

  • ICC/IF image of ab38281 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38281, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ketohexokinase staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab38281, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-ketohexokinase antibody (ab38281) at 1/100 dilution + Mouse kidney tissue lysate

    Predicted band size : 33 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 19 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Palavicino-Maggio CB & Kuzhikandathil EV Dietary Fructose and GLUT5 Transporter Activity Contribute to Antipsychotic-Induced Weight Gain. Schizophr Bull 42:1270-9 (2016). Read more (PubMed: 27056716) »
  • Della Casa L  et al. Effect of diets supplemented with different conjugated linoleic acid (CLA) isomers on protein expression in C57/BL6 mice. Genes Nutr 11:26 (2016). Read more (PubMed: 27713773) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Archilochus colubris Tissue lysate - whole (pectoralis, ankle flexors and extensors, liver, he)
Gel Running Conditions
Reduced Denaturing (10% gel, PVDF membrane)
Loading amount
10 µg
Specification
pectoralis, ankle flexors and extensors, liver, he
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Dr. Kenneth Welch

Verified customer

Submitted May 06 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Chicken Tissue lysate - whole (pectoralis)
Gel Running Conditions
Reduced Denaturing (10% gel, PVDF membrane)
Loading amount
10 µg
Specification
pectoralis
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Dr. Kenneth Welch

Verified customer

Submitted May 06 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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