Overview

  • Product name
    Anti-Kettin antibody [MAC 155]
  • Description
    Rat monoclonal [MAC 155] to Kettin
  • Specificity
    Antibody reacts with 500 and 700 kDa isoforms in Lethocerus and Drosophila flight and non-flight muscle. The epitope is near the middle of the molecule. Antibody reacts with Caenorhabditis (nematode) muscle and does not react with rabbit skeletal muscle.
  • Tested applications
    Suitable for: ICC/IF, Electron Microscopy, WBmore details
  • Species reactivity
    Reacts with: Caenorhabditis elegans, Drosophila melanogaster, Waterbug
    Does not react with: Rabbit
  • Immunogen

    Z-discs purified from Lethocerus indicus (Waterbug)

Properties

Applications

Our Abpromise guarantee covers the use of ab50585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
Electron Microscopy Use at an assay dependent dilution.
WB Use at an assay dependent concentration. PubMed: 22479211

Target

  • Function
    Key component in the assembly and functioning of adult and embryonic striated muscles and muscle tendons. By providing connections at the level of individual microfilaments, it contributes to the fine balance of forces between the two halves of the sarcomere. The size and extensibility of the cross-links are the main determinants of sarcomere extensibility properties of muscle. In non-muscle cells, seems to play a role in chromosome condensation and chromosome segregation during mitosis. Might link the lamina network to chromatin or nuclear actin, or both during interphase.
  • Tissue specificity
    Expressed in the mesoderm at stage 11, several hours before myoblast fusion, and persists in most muscle cells, somatic, visceral and pharyngeal muscles and their precursors, until the third instar. Also expressed in adult flight muscle Z-disks.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 7 fibronectin type-III domains.
    Contains 53 Ig-like (immunoglobulin-like) domains.
    Contains 1 SH3 domain.
    Contains 7 TPR repeats.
  • Developmental stage
    Expressed both maternally and zygotically.
  • Cellular localization
    Cytoplasm. Nucleus. Chromosome. Uniformly distributed along condensed mitotic chromosomes.
  • Information by UniProt
  • Database links
    • Alternative names
      • D Titin antibody
      • D-Titin antibody
      • Kettin antibody
      • Sallimus antibody
      • sls antibody
      • Titin antibody
      • TITIN_DROME antibody
      see all

    Images

    • Immunofluorescence analysis of Drosophila melanogaster larvae muscle, staining Kettin with ab50585.

    References

    This product has been referenced in:
    • Chechenova MB  et al. Functional redundancy and nonredundancy between two Troponin C isoforms in Drosophila adult muscles. Mol Biol Cell 28:760-770 (2017). IHC-Fr ; Drosophila melanogaster . Read more (PubMed: 28077621) »
    • Wójtowicz I  et al. Drosophila small heat shock protein CryAB ensures structural integrity of developing muscles, and proper muscle and heart performance. Development 142:994-1005 (2015). Read more (PubMed: 25715399) »

    See all 11 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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