Overview

  • Product name
  • Description
    Goat polyclonal to KGF
  • Specificity
    Based on ELISA and immunoblotting, this antibody shows less than 20% cross reactivity with recombinant human FGF9 and less than 10% cross reactivity with recombinant human FGF4.
  • Tested applications
    Suitable for: ELISA, Neutralising, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

  • General notes
    Endotoxin level is < 10 ng/mg antibody as determined by the LAL (Limulus amebocyte lysate) method.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Affinity isolated antigen specific antibody is obtained from goat anti-FGF7 antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Neutralising
WB
  • Application notes
    ELISA: Use at a concentration of 0.5 - 1.0 µg/ml.
    Neut: Use at a concentration of 0.1 - 100 µg/ml.
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 22.5 kDa.

    The Neutralization Dose 50 (ND50) for anti-human FGF7 (KGF) is approximately 6 to 12 µg/ml in the presence of 125 ng/ml of recombinant human FGF7 using the human 4MBr-5 cell line. The ND50 is the concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when the cytokine is present at a concentration just high enough to elicit a maximum response.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.
    • Tissue specificity
      Epithelial cell.
    • Sequence similarities
      Belongs to the heparin-binding growth factors family.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Form
      Potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells.
    • Alternative names
      • FGF 7 antibody
      • FGF-7 antibody
      • Fgf7 antibody
      • FGF7_HUMAN antibody
      • Fibroblast growth factor 7 antibody
      • HBGF 7 antibody
      • HBGF-7 antibody
      • HBGF7 antibody
      • Heparin binding growth factor 7 antibody
      • Heparin-binding growth factor 7 antibody
      • Keratinocyte growth factor antibody
      • KGF antibody
      see all

    References

    ab10423 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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