Overview

  • Product nameAnti-KGF antibody
    See all KGF primary antibodies
  • Description
    Rabbit polyclonal to KGF
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Sheep, Horse, Chicken, Dog, Pig, Mink (Mustela), Macaque Monkey, Deer, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human KGF.

  • Positive control
    • This antibody gave a positive signal in human skin. This antibody gave a positive result when used in the following methanol fixed cell lines: A431

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab90259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionGrowth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.
  • Tissue specificityEpithelial cell.
  • Sequence similaritiesBelongs to the heparin-binding growth factors family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • FormPotent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells.
  • Alternative names
    • FGF 7 antibody
    • FGF-7 antibody
    • Fgf7 antibody
    • FGF7_HUMAN antibody
    • Fibroblast growth factor 7 antibody
    • HBGF 7 antibody
    • HBGF-7 antibody
    • HBGF7 antibody
    • Heparin binding growth factor 7 antibody
    • Heparin-binding growth factor 7 antibody
    • Keratinocyte growth factor antibody
    • KGF antibody
    see all

Anti-KGF antibody images

  • ICC/IF image of ab90259 stained A431 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab90259 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • IHC image of KGF staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab90259, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References for Anti-KGF antibody (ab90259)

ab90259 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"