Overview

  • Product nameAnti-Ki67 antibody
    See all Ki67 primary antibodies
  • Description
    Rabbit polyclonal to Ki67
  • Tested applicationsSuitable for: IHC-FoFr, IHC-Fr, ICC/IF, WB, IP, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Ki67.

  • Positive control
    • Human Breast carcinoma and lymph node tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab66155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 19520159
IHC-Fr Use a concentration of 1.6 µg/ml.

See Abreview.

ICC/IF Use at an assay dependent concentration. PubMed: 20729478
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 359 kDa.
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionRequired to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.
  • Sequence similaritiesContains 1 FHA domain.
    Contains 16 K167R repeats.
    Contains 1 PP1-binding domain.
  • Developmental stageExpression occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected (at protein level) (PubMed:6206131). Present at highest level in G2 phase and during mitosis (at protein level). In interphase, forms fiber-like structures in fibrillarin-deficient regions surrounding nucleoli (PubMed:2674163, PubMed:8799815).
  • Post-translational
    modifications
    Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.
  • Cellular localizationChromosome. Nucleus. Nucleus, nucleolus. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106).
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen identified by monoclonal antibody Ki 67 antibody
    • Antigen identified by monoclonal antibody Ki-67 antibody
    • Antigen KI-67 antibody
    • Antigen KI67 antibody
    • Antigen Ki67 antibody
    • KI67_HUMAN antibody
    • KIA antibody
    • Marker of proliferation Ki-67 antibody
    • MIB 1 antibody
    • MIB antibody
    • MKI67 antibody
    • PPP1R105 antibody
    • Proliferation marker protein Ki-67 antibody
    • Proliferation related Ki 67 antigen antibody
    • Protein phosphatase 1 regulatory subunit 105 antibody
    • RP11-380J17.2 antibody
    see all

Anti-Ki67 antibody images

  • Immunohistochemical analysis of formaldehyde fixed mouse skin sections labelling Ki67 with ab66155 at a dilution of 1:1000. The secondary antibody used was a goat anti-rabbit IgG (HRP). Antigen retrieval was performed using Citrate buffer pH 6.0 for 20 minutes at 97ºC.

    See Abreview

  • ab66155 staining Ki67 in human LnCAP cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with ethanol and triton, and blocked for 1 hour at 26°C. Samples were incubated with primary antibody (1/200 in blocking buffer) for 16 hours at 4°C. An undiluted IRDye® 800CW-conjugated goat anti-rabbit IgG (H+L) polyclonal was used as the secondary antibody.

    See Abreview

  • ab66155 staining Ki67 in murine brain tissue by Immunohistochemistry (Frozen sections). Tissue was fixed with paraformaldehyde and then blocked with 5% serum for 1 hour at 25°C followed by incubation with the primary antibody at a 1/200 dilution for 12 hours at 4°C. ab96919 Donkey polyclonal to anti-rabbit DyLight® 488 (IgG - H&L), pre-adsorbed was used as the secondary antibody undiluted.

    See Abreview

  • ab66155 at 1/300 dilution staining Ki67 in rat glioma tissue by immunohistochemistry (frozen sections). Sections were paraformaldehyde fixed prior to blocking in 1% BSA for 30 minutes at 20°C and then incubated with ab66155 for 18 hours at 4°C.

    See Abreview

  • This image shows a human lymph node stained with ab66155 at 1/200 dilution for 10 min at RT.
  • ab66155 staining ki67 in proliferating mouse astrocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldahyde, permeabilized with 0.3% TritonX 100 and 3M Glycine and blocked with 10% BSA for 20 minutes at 20°C. Samples were incubated with primary antibody (1/250 in 4% BSA/PBS) for 18 hours at 4°C. ab150073 at a dilution of 1/500 was used as the secondary antibody.

    Astrocytes (orange) labelled with Ki67 proliferation marker (violet) and counter stained with DAPI (blue).

    Scale bar 20um, magnification x40 oil.

References for Anti-Ki67 antibody (ab66155)

This product has been referenced in:
  • Su B  et al. Diallyl disulfide suppresses epithelial-mesenchymal transition, invasion and proliferation by downregulation of LIMK1 in gastric cancer. Oncotarget 7:10498-512 (2016). Read more (PubMed: 26871290) »
  • Wang TC  et al. Characterization of highly proliferative secondary tumor clusters along host blood vessels in malignant glioma. Mol Med Rep 12:6435-44 (2015). IHC-Fr ; Rat . Read more (PubMed: 26299849) »

See all 34 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6
Permeabilization Yes - 0.3 % Triton X-100
Specification Brain
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10 · Temperature: 25°C
Fixative Paraformaldehyde
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Submitted May 15 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Syrian Hamster Tissue sections (skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrat-Buffer pH 6,0
Permeabilization No
Specification skin
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
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Verified customer

Submitted Jan 22 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (jejunum)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrat-Buffer pH 6,0
Permeabilization No
Specification jejunum
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
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Submitted Jan 22 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Proliferating Astrocytes)
Permeabilization Yes - 0.3% TritonX 100 and 3M Glycine
Specification Proliferating Astrocytes
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative Paraformaldehyde
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Verified customer

Submitted Nov 23 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Spleen)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Spleen
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Sep 29 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Marmoset (common) Tissue sections (Colon)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Colon
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Sep 25 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer ph6 20 min at 97ºC IN A PT LINK
Permeabilization No
Specification Skin
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
Username

Miss. Virginia Gutiérrez

Verified customer

Submitted Sep 24 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Tonsil)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Tonsil
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Aug 20 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
Sample Rat Cell (vascular smooth muscle cell culture)
Specification vascular smooth muscle cell culture
Permeabilization Yes - Triton X100, 0.3% w/v
Fixative Paraformaldehyde
Username

Dr. Maksym Harhun

Verified customer

Submitted Mar 31 2015

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (7)
Sample Human Cell lysate - whole cell (Human Prostate Cell Line LNCaP)
Specification Human Prostate Cell Line LNCaP
Treatment Media vs Activin A 50 ng/ml
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Username

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Submitted Feb 16 2015

1-10 of 21 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"