Overview

  • Product nameAnti-Ki67 antibody
    See all Ki67 primary antibodies
  • Description
    Rabbit polyclonal to Ki67
  • Tested applicationsSuitable for: ICC/IF, ICC, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human, African Green Monkey
  • Immunogen

    Synthetic peptide corresponding to Human Ki67. Immunogen is from the 62 base pair region of the human Ki-67 antigen.
    Database link: P46013

  • Positive control
    • Tonsil.

Properties

Applications

Our Abpromise guarantee covers the use of ab833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/400. PubMed: 18635871
ICC Use at an assay dependent concentration.
IHC-P 1/25 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. We recommend using an ABC system for detection of this antibody.
IHC-Fr 1/25 - 1/50. 1/25 - 1/50. We suggest an incubation period of 30 minutes at room temperature and to use DAB to stain the protein.

Target

  • FunctionThought to be required for maintaining cell proliferation.
  • Sequence similaritiesContains 1 FHA domain.
  • Developmental stageExpression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected.
  • Cellular localizationNucleus. Nucleus, nucleolus. Chromosome. Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen identified by monoclonal antibody Ki 67 antibody
    • Antigen KI-67 antibody
    • Antigen KI67 antibody
    • KI67_HUMAN antibody
    • KIA antibody
    • Marker of proliferation Ki-67 antibody
    • MIB 1 antibody
    • MIB antibody
    • MKI67 antibody
    • PPP1R105 antibody
    • Proliferation related Ki 67 antigen antibody
    • Protein phosphatase 1 regulatory subunit 105 antibody
    • RP11-380J17.2 antibody
    see all

Anti-Ki67 antibody images

  • ab833 - immunohistochemistry

    Formalin fixed paraffin embedded human tonsil stained with Ki-67, using ABC and AEC chromogen.

  • HT-29 xenograft tumor in mouse 20x pretreatment w/ citrate pH 6.0 using anti-Ki67 antibody ab833 at 5 ug/mL for 30 min at RT.
  • Immunohistochemical analysis of Human skin tissue, staining Ki67 (red) with ab833.

    Antigen retrieval was performed by heat mediation in a EDTA/Tris buffer (pH 8.0). Sections were blocked with 10% NGS for 1 hour at room temperature, followed by incubation with primary antibody (1/25). An AlexFluor®546 anti-rabbit IgG (1/500) was used as the secondary antibody.

References for Anti-Ki67 antibody (ab833)

This product has been referenced in:
  • Buchholz M  et al. A multistep high-content screening approach to identify novel functionally relevant target genes in pancreatic cancer. PLoS One 10:e0122946 (2015). ICC . Read more (PubMed: 25849100) »
  • Xu H  et al. A puromycin selectable cell line for the enrichment of mouse embryonic stem cell-derived V3 interneurons. Stem Cell Res Ther 6:220 (2015). ICC/IF . Read more (PubMed: 26555777) »

See all 57 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Marmoset (common) Tissue sections (Colon)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Colon
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Sep 25 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Tonsil)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Tonsil
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Aug 20 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (prostate cancer)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.05% trypsin for 10 min at 37°C
Permeabilization No
Specification prostate cancer
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 21 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Sample African Green Monkey Cell (Cos7 Monkey Kidney Fibroblast)
Specification Cos7 Monkey Kidney Fibroblast
Permeabilization Yes - .3% Triton X100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 13 2013

>As I mentioned previously, I would recommend running a no primary antibody control to ensure that the diffuse nuclear staining cannot be attributed to non-specific binding. If the no primary antibody control is clear, then I would conclude thatmost of...

Read More

Ki67 will be localized in the perinucleolar region in the G1 phase and in the nuclear matrix (your hazy nuclear stain) in the later S, G2, and M phases. During G0, Ki67 will not be detected. If you are looking to quantify interphase / division vs quies...

Read More
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Adult skin)
Specification Adult skin
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric buffer, pH 6
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Apr 06 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Specification Liver
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 20 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (retinoblastoma cells)
Specification retinoblastoma cells
Fixative Cytospin solution: methanol, acetone, glycerine
Permeabilization Yes - PBS-Tween
Username

Abcam user community

Verified customer

Submitted Apr 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Liver)
Specification Liver
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Blocking step Serum as blocking agent for 5 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Jan 18 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"