Overview

  • Product nameAnti-Ki67 antibody [B126.1]See all Ki67 primary antibodies ...
  • Description
    Mouse monoclonal [B126.1] to Ki67
  • SpecificityThis antibody reacts with an epitope on a nuclear antigen Ki-67, which is expressed in cycling cells (G1, S, G2, Mphases). It is a marker of native Ki-67 and recombinant fragments of Ki-67. The specificity and reactivity of this clone is equal or superior to any other available Ki-67 monoclonal antibody. Another proliferation cell nuclear antibody[PCNA], ab29, produces far superior staining profiles than any of the Ki-67 monoclonals we have tested.
  • Tested applicationsFlow Cyt, IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Other Immunogen Type corresponding to Human Ki67. Raised against nuclear fractions of human tumor cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab8191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent dilution.
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The antibody gave good results after 5 minutes of heat-mediated retrieval in a microwave oven.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionThought to be required for maintaining cell proliferation.
  • Sequence similaritiesContains 1 FHA domain.
  • Developmental stageExpression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected.
  • Cellular localizationNucleus. Nucleus > nucleolus. Chromosome. Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen identified by monoclonal antibody Ki 67 antibody
    • Antigen KI-67 antibody
    • Antigen KI67 antibody
    • KI67_HUMAN antibody
    • KIA antibody
    • MKI67 antibody
    • Proliferation related Ki 67 antigen antibody
    • RP11-380J17.2 antibody
    see all

Anti-Ki67 antibody [B126.1] images

  • IHC-P image of ab8191 (1/50) staining human melanoma tumour sections. Secondary antibody: rabbit polyclonal to mouse IgG conjugated to HRP (ab6728).

    See Abreview

  • ab8191 at 1/100 staining normal human primary fibroblasts by ICC/IF. The cells were formaldehyde fixed, permeabilized and fixed before incabation with the antibody for 2 hours at 4°C. An Alexa Fluor ® 488 conjugated donkey polycloanl was used as the secondary.

    See Abreview

References for Anti-Ki67 antibody [B126.1] (ab8191)

This product has been referenced in:
  • Liu C  et al. Prickle1 is expressed in distinct cell populations of the central nervous system and contributes to neuronal morphogenesis. Hum Mol Genet N/A:N/A (2013). Mouse . Read more (PubMed: 23420014) »
  • Liu C  et al. Regulation of retinal progenitor expansion by Frizzled receptors: implications for microphthalmia and retinal coloboma. Hum Mol Genet 21:1848-60 (2012). IHC (Methanol fixed) ; Mouse . Read more (PubMed: 22228100) »

See all 9 Publications for this product

Product Wall

DISCOUNT CODE: ***
Expiration date: December 22, 2012

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forab8191 with rat samplesand include this code in the &ldq...

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Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Normal Primary Fibroblast)
Specification Normal Primary Fibroblast
Fixative Formaldehyde
Permeabilization Yes - TritonX100
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jul 24 2007

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Melanoma tumors)
Specification Melanoma tumors
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Username

Mrs. Olga Bragina

Verified customer

Submitted Aug 03 2006

Thank you for your enquiry. This antibody should not produce staining in neutrophils. The staining you see may be due to endogenous peroxidase present in neutrophils. Have you tried blocking endogenous peroxidase with H2O2? The protocol we reco...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"