RabMAb

Anti-Ki67 antibody [EPR3611] (Alexa Fluor® 488) (ab154201)

Overview

  • Product nameAnti-Ki67 antibody [EPR3611] (Alexa Fluor® 488)See all Ki67 primary antibodies ...
  • Description
    Rabbit monoclonal [EPR3611] to Ki67 (Alexa Fluor® 488)
  • ConjugationAlexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applicationsFlow Cyt, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to amino acids within Human Ki67

  • Positive control
    • HeLa cells
  • General notesThe Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
    Produced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab154201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/100.
ICC 1/50 - 1/100.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionThought to be required for maintaining cell proliferation.
  • Sequence similaritiesContains 1 FHA domain.
  • Developmental stageExpression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected.
  • Cellular localizationNucleus. Nucleus > nucleolus. Chromosome. Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen identified by monoclonal antibody Ki 67 antibody
    • Antigen KI-67 antibody
    • Antigen KI67 antibody
    • KI67_HUMAN antibody
    • KIA antibody
    • MKI67 antibody
    • Proliferation related Ki 67 antigen antibody
    • RP11-380J17.2 antibody
    see all

Anti-Ki67 antibody [EPR3611] (Alexa Fluor® 488) images

  • Flow cytometry of HeLa cells labelling Ki67 with ab154201 at 1/100 dilution (green) or negative control (red).
  • Immunofluorescence of HeLa cells staining Ki67 (green) with ab154201 at 1/50 dilution with Alexa Fluor® 488. The image on the right is a DAPI only control.

References for Anti-Ki67 antibody [EPR3611] (Alexa Fluor® 488) (ab154201)

ab154201 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X-100 in PBS
Username

Dr. Kirk McManus

Verified customer

Submitted Feb 19 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"