The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
Use neat. Note: samples are acetone fixed.
FunctionThought to be required for maintaining cell proliferation.
Sequence similaritiesContains 1 FHA domain.
Developmental stageExpression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected.
Cellular localizationNucleus. Nucleus, nucleolus. Chromosome. Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.