Anti-KIAA0020 antibody (ab156692)
Key features and details
- Rabbit polyclonal to KIAA0020
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KIAA0020 antibody -
Description
Rabbit polyclonal to KIAA0020 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Macaque monkey, Rhesus monkey, Gorilla, Common marmoset -
Immunogen
Synthetic peptide corresponding to a region within amino acids 1-50 of Human KIAA0020 (NP_055693.4).
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Positive control
- HeLa whole cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab156692 was affinity purified using an epitope specific to KIAA0020 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156692 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 74 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 74 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Tissue specificity
Widely expressed. -
Sequence similarities
Contains 1 PUM-HD domain.
Contains 6 pumilio repeats. -
Domain
The HA-8 region can be cleaved and exposed at the cell surface where it plays a role as a minor histocompatibility HLA-A*0201-restricted antigen. -
Cellular localization
Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 464968 Chimpanzee
- Entrez Gene: 100403964 Common marmoset
- Entrez Gene: 101140298 Gorilla
- Entrez Gene: 9933 Human
- Entrez Gene: 696063 Rhesus monkey
- GenBank: NP_055693.4 Human
- Omim: 609960 Human
- SwissProt: Q15397 Human
see all -
Alternative names
- HA-8 antibody
- HBV X transactivated gene 5 protein antibody
- HBV X-transactivated gene 5 protein antibody
see all
Images
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All lanes : Anti-KIAA0020 antibody (ab156692) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 74 kDa
Exposure time: 3 minutes -
Detection of KIAA0020 by Western blot of immunoprecipitate.
Immunoprecipitation of KIAA0020 (6 µg of antibody per mg of HeLa whole cell lysate; 1 mg/IP; 20% of IP loaded/lane), using the following:
Lane 1: ab156692
Lane 2: alternative anti-KIAA0020 antibody
Lane 3: Control IgG
For the subsequent Western blot of immunoprecipitates, ab156692 was used at 1 µg/ml.
Detection: Chemiluminescence with exposure time of 3 minutes.
Lane 1 : Anti-KIAA0020 antibody (ab156692) at 1 µg/ml
Lane 2 : Alternative rabbit anti-KIAA0020 antibody
Lane 3 : Control IgG
All lanes : HeLa whole cell lysate
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab156692 has not yet been referenced specifically in any publications.