Overview

  • Product name
  • Description
    Rabbit polyclonal to KIAA0319
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Cow, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 151-200 (EEMSEYSDDY RELEKDLLQP SGKQEPRGSA EYTDWGLLPG SEGAFNSSVG) of Human KIAA0319 (EAW55455).

  • Positive control
    • HepG2 cell lysate; Human kidney tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Constituents: 97% PBS, 2% Sucrose
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 56 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Function
    Involved in neuronal migration during development of the cerebral neocortex. May function in a cell autonomous and a non-cell autonomous manner and play a role in appropriate adhesion between migrating neurons and radial glial fibers. May also regulate growth and differentiation of dendrites.
  • Tissue specificity
    Detected in adult brain cortex and fetal frontal lobe (at protein level). Highly expressed in brain cortex, putamen, amygdala, hippocampus and cerebellum.
  • Involvement in disease
    Defects in KIAA0319 may be a cause of susceptibility to dyslexia type 2 (DYX2) [MIM:600202]; also known as specific reading disability type 2. Dyslexia is a relatively common, complex cognitive disorder that affects 5% to 10% of school-aged children. The disorder is characterized by an impairment of reading performance despite adequate motivational, educational and intellectual opportunities and in the absence of sensory or neurological disability. Note=A lower expression is associated with the risk haplotype.
  • Sequence similarities
    Contains 1 MANSC domain.
    Contains 5 PKD domains.
  • Developmental stage
    Expressed in the developing cerebral neocortex and glanglionic eminence in 57 days post-fertilization fetal brain.
  • Post-translational
    modifications
    N-glycosylated.
    O-glycosylated.
    Shedding of the extracellular domain and intramembrane cleavage produce several proteolytic products. The intramembrane cleavage releases a soluble cytoplasmic polypeptide that translocates to the nucleolus.
  • Cellular localization
    Cell membrane. Early endosome membrane. Low-abundance isoforms lacking the transmembrane domain have been described; these are secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • DLX 2 antibody
    • DLX2 antibody
    • DYLX 2 antibody
    • DYLX2 antibody
    • Dyslexia susceptibility 2 antibody
    • Dyslexia-associated protein KIAA0319 antibody
    • DYX 2 antibody
    • DYX2 antibody
    • K0319_HUMAN antibody
    • Kiaa0319 antibody
    • MGC176717 antibody
    see all

Images

  • Anti-KIAA0319 antibody (ab118923) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Predicted band size : 56 kDa
  • ab118923, at 8 µg/ml, staining KIAA0319 in paraffin embedded Human kidney tissue by Immunohistochemistry.
    Cells with positive label are epithelial cells of renal tubules (indicated by arrows).

References

ab118923 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing (4-10 % gradient gel)
Loading amount
100 µg
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

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Verified customer

Submitted Jul 21 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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