• Product nameAnti-KIAA0427 antibody
    See all KIAA0427 primary antibodies
  • Description
    Rabbit polyclonal to KIAA0427
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 36 - 85 (QVQGLLADKT EGDGESERTQ SHISQWTADC SEPLDSSCSF SRGRAPPQQN) of Human KIAA0427 (NP_055587).

  • Positive control
    • HepG2 cell lysate.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab82891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/12500.


  • FunctionSpecifically required for the pioneer round of mRNA translation mediated by the cap-binding complex (CBC), that takes place during or right after mRNA export via the nuclear pore complex (NPC). Acts via its interaction with the NCBP1/CBP80 component of the CBC complex and recruits the 40S small subunit of the ribosome via eIF3. In contrast, it is not involved in steady state translation, that takes place when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. Also required for nonsense-mediated mRNA decay (NMD), the pioneer round of mRNA translation mediated by the cap-binding complex playing a central role in nonsense-mediated mRNA decay (NMD).
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the CTIF family.
    Contains 1 MIF4G domain.
  • Cellular localizationCytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • CBP80/20-dependent translation initiation factor antibody
    • ctif antibody
    • CTIF_HUMAN antibody
    • Gm672 antibody
    • Hypothetical protein LOC9811 antibody
    • Uncharacterized protein KIAA0427 antibody
    see all

Anti-KIAA0427 antibody images

  • Anti-KIAA0427 antibody (ab82891) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 68 kDa
    Observed band size : 68 kDa
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

References for Anti-KIAA0427 antibody (ab82891)

ab82891 has not yet been referenced specifically in any publications.

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I am sorry to hear that you have been having issues with these antibodies. Both ab82891 and ab99060 are covered by our Abpromise guarantee. According to Abpromise guarantee, since these products has been purchased within the last six months, we ar...

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