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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration information loading...
Purity Immunogen affinity purified
Purification notes Antibody was affinity purified using an epitope specific to KIAA0460 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 155 kDa).
Use at an assay dependent dilution.
Relevance This is a hypothetical protein fragment believed to be involved in DNA repair.
KIAA0460 contains 1 CID domain.
Cellular localization Nuclear
FLJ 32145 antibody
HSPC 099 antibody
Anti-KIAA0460 antibody images
Western blot - KIAA0460 antibody (ab17996)
Lanes 1 - 2 : Anti-KIAA0460 antibody (ab17996) at 1 µg/ml Lanes 3 - 4 : Anti-KIAA0460 antibody (ab17996) at 0.4 µg/ml Lane 1 : Whole cell lysate from HEK239 cells at 15 µg Lane 2 : Whole cell lysate from HEK239 cells at 50 µg Lane 3 : Whole cell lysate from HEK239 cells at 15 µg Lane 4 : Whole cell lysate from HEK239 cells at 50 µg Predicted band size : 155 kDa Observed band size : 225 kDa ( why is the actual band size different from the predicted?) Exposure time : 1 minute
References for Anti-KIAA0460 antibody (ab17996)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"