Overview

  • Product name
  • Description
    Goat polyclonal to KIAA0460
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide:

    FKNMLKNASRKPSDDKHFGQA

    , corresponding to amino acids 1000-1050 of Human KIAA0460.

  • Positive control
    • HEK293 cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antibody was affinity purified using an epitope specific to KIAA0460 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 155 kDa).
IP Use at an assay dependent dilution.

Target

  • Relevance
    This is a hypothetical protein fragment believed to be involved in DNA repair. KIAA0460 contains 1 CID domain.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • FLJ 32145 antibody
    • HSPC 099 antibody
    • HSPC099 antibody
    • Hypothetical Protein DKFZp686H22247 antibody
    • Hypothetical protein KIAA0460 antibody
    • KIAA 0460 antibody
    • Regulation of nuclear pre mRNA domain containing protein 2 antibody
    • Regulation of nuclear pre-mRNA domain containing 2 antibody
    • RPRD 2 antibody
    • RPRD2 antibody
    see all

Images

  • Lanes 1 - 2 : Anti-KIAA0460 antibody (ab17996) at 1 µg/ml
    Lanes 3 - 4 : Anti-KIAA0460 antibody (ab17996) at 0.4 µg/ml

    Lane 1 : Whole cell lysate from HEK239 cells at 15 µg
    Lane 2 : Whole cell lysate from HEK239 cells at 50 µg
    Lane 3 : Whole cell lysate from HEK239 cells at 15 µg
    Lane 4 : Whole cell lysate from HEK239 cells at 50 µg


    Predicted band size : 155 kDa
    Observed band size : 225 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

References

ab17996 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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