Overview

  • Product name
  • Description
    Rabbit polyclonal to KIAA0460
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within the middle amino acids 1332-1381 (LQGTLAEHFG VLPGPRDHGG PTQRDLNGPG LSRVRESLTL PSHSLEHLGP) of human KIAA0460 (NP_056018).

  • Positive control
    • HepG2 cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified by peptide affinity chromatography method.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 156 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    This is a hypothetical protein fragment believed to be involved in DNA repair. KIAA0460 contains 1 CID domain.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • FLJ 32145 antibody
    • HSPC 099 antibody
    • HSPC099 antibody
    • Hypothetical Protein DKFZp686H22247 antibody
    • Hypothetical protein KIAA0460 antibody
    • KIAA 0460 antibody
    • Regulation of nuclear pre mRNA domain containing protein 2 antibody
    • Regulation of nuclear pre-mRNA domain containing 2 antibody
    • RPRD 2 antibody
    • RPRD2 antibody
    see all

Images

  • Anti-KIAA0460 antibody (ab83823) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 156 kDa
    Observed band size : 156 kDa
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

References

ab83823 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab83823.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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