Overview

  • Product nameAnti-KIAA0513 antibody
    See all KIAA0513 primary antibodies
  • Description
    Rabbit polyclonal to KIAA0513
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    N terminal amino acids 7-123 of Human KIAA0513.

  • Positive control
    • Human skeletal muscle tissue; RT-4 and Human liver tissue lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Tissue specificityWidely expressed, highest levels in cerebellum, brain cortex, hippocampus, pons, putamen and amygdala. Highly expressed in neurons, but also present in glial cells. Slightly higher expression in the dorsolateral prefrontal cortex of schizophrenic patients compared to control individuals.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • K0513_HUMAN antibody
    • KIAA0513 antibody
    • Uncharacterized protein KIAA0513 antibody

Anti-KIAA0513 antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in plasma membrane and cytoplasm. Recommended concentration of ab121430 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121430, at a 1/45 dilution, staining KIAA0513 in paraffin-embedded Human skeletal muscle, by Immunohistochemistry.
  • All lanes : Anti-KIAA0513 antibody (ab121430) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma
    Lane 4 : Human Liver tissue lysate
    Lane 5 : Human Liver tissue lysate

    Developed using the ECL technique

References for Anti-KIAA0513 antibody (ab121430)

ab121430 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"