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Rabbit polyclonal to KIAA0753
antigen sequence, corresponding to amino acids 850-961 of Human KIAA0753. (Q2KHM9)
Human gall bladder tissue; RT 4, U 51 MG, Human plasma and tonsil lysates; human cell line A-431
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/250 - 1/500.
Information by UniProt
Uncharacterized protein KIAA0753 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIAA0753 antibody (ab121736)
ab121736 at 1/150 dilution staining KIAA0753 in Paraffin-embedded Human gall bladder tissue by Immunohistochemistry.
Western blot - Anti-KIAA0753 antibody (ab121736)
All lanes : Anti-KIAA0753 antibody (ab121736) at 1/250 dilution Lane 1 : RT 4 lysate Lane 2 : U 251 MG lysate Lane 3 : Human plasma lysate Lane 4 : Human liver lysate Lane 5 : Human tonsil lysate Developed using the ECL technique
Immunocytochemistry/ Immunofluorescence - Anti-KIAA0753 antibody (ab121736)
Immunofluorescent staining of Human cell line A-431 using 4 µg/ml ab121736 (green).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-KIAA0753 antibody(ab121736)
Immunohistochemical staining of Human gall bladder shows strong cytoplasmic positivity in glandular cells using ab121736
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