Overview

  • Product name
    Anti-KIAA0753 antibody
  • Description
    Rabbit polyclonal to KIAA0753
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 850-961 of Human KIAA0753. (Q2KHM9)

  • Positive control
    • Human gall bladder tissue; RT 4, U 51 MG, Human plasma and tonsil lysates; human cell line A-431

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Images

  • ab121736 at 1/150 dilution staining KIAA0753 in Paraffin-embedded Human gall bladder tissue by Immunohistochemistry.
  • All lanes : Anti-KIAA0753 antibody (ab121736) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique
  • Immunofluorescent staining of Human cell line A-431 using 4 µg/ml ab121736 (green).
  • Immunohistochemical staining of Human gall bladder shows strong cytoplasmic positivity in glandular cells using ab121736

References

ab121736 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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