Overview

  • Product name
  • Description
    Rabbit polyclonal to KIAA0802
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A region between amino acids 926-976 of Human KIAA0802 (AAH40542.2).

  • Positive control
    • HeLa lysate
  • General notes
    Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab100914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 209 kDa.
IP Use at 5-15 µg/mg of lysate.

Target

  • Function
    Microtubule-associated factor involved in the late phase of epithelial polarization and microtubule dynamics regulation. Plays a role in the development and maintenance of non-centrosomal microtubule bundles at the lateral membrane in polarized epithelial cells.
  • Sequence similarities
    Belongs to the SOGA family.
  • Cellular localization
    Lateral cell membrane. Apical cell membrane. Cytoplasm, cytoskeleton, spindle pole. Midbody. Cytoplasm, cytoskeleton. Colocalized with microtubules at the base of cilia. Gradually accumulates on the apicobasal microtubule bundles during epithelial cell polarization (By similarity). Colocalized with the apicobasal microtubule bundles running beneath the lateral membrane. Colocalized with microtubule bundles in the spindle pole in mitotic cells and in the midbodies at the end of cytokinesis.
  • Information by UniProt
  • Database links
  • Alternative names
    • Coiled-coil domain-containing protein 165 antibody
    • Microtubule cross-linking factor 1 antibody
    • MTCL1 antibody
    • MTCL1_HUMAN antibody
    • PAR-1-interacting protein antibody
    • SOGA family member 2 antibody
    see all

Images

  • All lanes : Anti-KIAA0802 antibody (ab100914) at 1 µg/ml

    Lane 1 : HeLa Whole Cell Lysate at 50 µg
    Lane 2 : HeLa Whole Cell Lysate at 15 µg
    Lane 3 : HeLa Whole Cell Lysate at 5 µg


    Predicted band size : 209 kDa


    Exposure time : 3 minutes
  • ab100914 at 1 µg/ml staining KIAA0802 in HeLa cell lysate immunoprecipitated using ab100914 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Exposure time: 3 minutes

References

ab100914 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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