Anti-METTL13 antibody (ab186002)
Key features and details
- Rabbit polyclonal to METTL13
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-METTL13 antibody
See all METTL13 primary antibodies -
Description
Rabbit polyclonal to METTL13 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan -
Immunogen
Synthetic peptide within Human METTL13. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_057019.3
Database link: Q8N6R0 -
Positive control
- HeLa, 293T or Jurkat lysate
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186002 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
Use at 2-10 µg/mg of lysate.
|
|
WB |
1/1000 - 1/5000. Predicted molecular weight: 79 kDa.
|
Notes |
---|
IP
Use at 2-10 µg/mg of lysate. |
WB
1/1000 - 1/5000. Predicted molecular weight: 79 kDa. |
Target
-
Sequence similarities
Belongs to the methyltransferase superfamily. - Information by UniProt
-
Database links
- Entrez Gene: 456446 Chimpanzee
- Entrez Gene: 51603 Human
- Entrez Gene: 100432314 Orangutan
- SwissProt: Q8N6R0 Human
- Unigene: 494705 Human
-
Alternative names
- 5630401D24Rik antibody
- CGI-01 antibody
- MET13_HUMAN antibody
see all
Images
-
Detection of Human METTL13 by Western Blot of Immunoprecipitates. 1 mg for IP; 20% of IP HeLa whole cell lysate loaded. ab186002 used for IP at 1 µg/ml. For blotting immunoprecipitated METTL13, ab186002 was used at 1 µg/ml.
-
All lanes : Anti-METTL13 antibody (ab186002) at 0.4 mg/ml
Lane 1 : HeLa lysate
Lane 2 : 293T lysate
Lane 3 : Jurkat lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 79 kDa
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab186002 has been referenced in 3 publications.
- Wu Q et al. METTL13 facilitates cell growth and metastasis in gastric cancer via an eEF1A/HN1L positive feedback circuit. J Cell Commun Signal 17:121-135 (2023). PubMed: 35925508
- Liu Z et al. METTL13 inhibits progression of clear cell renal cell carcinoma with repression on PI3K/AKT/mTOR/HIF-1a pathway and c-Myc expression. J Transl Med 19:209 (2021). PubMed: 33985542
- Cen S et al. TRAF4 acts as a fate checkpoint to regulate the adipogenic differentiation of MSCs by activating PKM2. EBioMedicine 54:102722 (2020). PubMed: 32268273