Overview

  • Product nameAnti-KIAA1045 antibody
    See all KIAA1045 primary antibodies
  • Description
    Rabbit polyclonal to KIAA1045
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    antigen sequence: LLTEEEMYSL TETFQRCKVI PDCSLTLEDF LRYRHQAAKR GDRDRALSEE QEEQAARQFA ALDPEHRGHI EWPDFLSHES LLLLQQLR, corresponding to amino acids 194-281 of Human KIAA1045.

  • Positive control
    • RT4, U 251 MG, Human Liver and Human Tonsil lysates; Human pancreas tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Anti-KIAA1045 antibody images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab121643 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
    Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
  • All lanes : Anti-KIAA1045 antibody (ab121643) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate
    10-20% SDS PAGE
  • ab121643 at 1/500 dilution staining KIAA1045 in Paraffin-embedded Human pancreas tissue by Immunohistochemistry.

References for Anti-KIAA1045 antibody (ab121643)

ab121643 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab121643.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"